A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis

• Published in: Carbohydrate polymers Vol. 124; pp. 90 - 97
• Main Authors: Mei, Yuxia, Zhu, Hai, Hu, Qiming, Liu, Yangyang, Zhao, Shumiao, Peng, Nan, Liang, Yunxiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 25.06.2015
• Subjects: Animals; antineoplastic activity; antineoplastic agents; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - therapeutic use; Antitumor activity; Apoptosis; Apoptosis - drug effects; cellulose; Fourier transform infrared spectroscopy; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - therapeutic use; gas chromatography-mass spectrometry; methylation; Mice; mycelium; Mycelium - chemistry; neoplasm cells; nuclear magnetic resonance spectroscopy; oxidation; Phellinus linteus; Plant Extracts; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - therapeutic use; Sarcoma 180 - drug therapy; Structural analysis; Phellinus linteus; Antitumor activity; Polysaccharides; Structural analysis; Apoptosis
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2015.02.009

•PLPS-1 and -2 were purified from mycelia of Phellinus linteus.•The structures of the two polysaccharides were elucidated to be quite different.•Only PLPS-1 displayed anti-proliferative effect against S-180 cells.•PLPS-1 induced apoptosis of S-180 cells in a dose-dependent manner.•The difference in antitumor activity results from the structural differences. Two novel polysaccharides termed PLPS-1 and PLPS-2 were isolated from mycelia of cultured Phellinus linteus by hot water extraction, purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, and structurally characterized by FTIR and NMR spectroscopy, GC–MS, periodate oxidation/Smith degradation, and methylation analysis. The monosaccharide compositions of PLPS-1 (MW 2.5×105Da) and PLPS-2 (MW 2.8×104Da) were respectively Glc, Ara, Fuc, Gal, and Xyl in molar ratio 21.964:1.336:1.182:1:1, and Glc, Gal, Man, Ara, Fuc, Xyl in molar ratio 14.368:2.594:1.956:1.552:1.466:1; i.e., both were heteropolysaccharides. The backbone of PLPS-1 consisted primarily of repeating α-d-Glc(1→4)-α-d-Glc(1→6) units, while that of PLPS-2 consisted of α-(1→3)-d-Glc and α-(1→6)-d-Glc. The side branches were also different in their carbohydrate components. In in vitro antitumor assays, PLPS-1 displayed strong anti-proliferative effect against S-180 sarcoma cells through apoptosis, whereas PLPS-2 had no such effect. The difference in antitumor activity between the two PLPS evidently results from their structural differences. PLPS-1 has potential as a novel anticancer agent.