Protective immunity against lethal anaphylactic reaction in Toxoplasma gondii -infected mice by DNA vaccination with T. gondii -derived heat shock protein 70 gene

• Published in: Parasitology international Vol. 59; no. 2; pp. 105 - 111
• Main Authors: Kikumura, Akitoshi, Fang, Hao, Mun, Hye-Seong, Uemura, Noriko, Makino, Masayuki, Sayama, Yusuke, Norose, Kazumi, Aosai, Fumie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.06.2010
• Subjects: Anaphylactic reaction; Anaphylaxis - immunology; Anaphylaxis - prevention & control; Animals; Antigens, Protozoan - genetics; Antigens, Protozoan - immunology; DNA, Protozoan - genetics; DNA, Protozoan - immunology; Female; Gastroenterology and Hepatology; HSP70 Heat-Shock Proteins - administration & dosage; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - immunology; Infectious Disease; Mice; Mice, Inbred C57BL; Protozoan Proteins - genetics; Protozoan Proteins - immunology; Protozoan Vaccines - administration & dosage; Protozoan Vaccines - genetics; Protozoan Vaccines - immunology; T.g.HSP70 gene vaccine; Toxoplasma; Toxoplasma - immunology; Toxoplasma gondii; Toxoplasmosis, Animal - immunology; Toxoplasmosis, Animal - prevention & control; Vaccination - methods; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; T.g.HSP70 gene vaccine; Toxoplasma gondii; Anaphylactic reaction
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2010.03.006

Abstract Toxoplasma gondii -derived heat shock protein 70 ( T.g. HSP70) was proven to induce lethal anaphylactic reaction in T. gondii -infected mice through platelet-activating factor (PAF)-mediated, but not classical IgE-dependent, pathway via TLR4/MyD88 signal pathway. The effector cells generating PAF and causing T.g. HSP70-induced anaphylactic reaction were CD11b+ and CD11c+ cells, although the reaction was enhanced by marked IFN-γ production by CD11b+ , CD11c+ , CD4+ and CD8+ splenocytes. In the present study, the effects of T.g. HSP70 gene vaccine targeting peripheral dendritic cells were evaluated against T.g. HSP70-induced anaphylactic reaction in T. gondii -infected mice. C57BL/6 mice receiving T.g. HSP70 gene vaccine showed prolonged survival. Platelets of peripheral blood, which completely disappeared during the T.g. HSP70-induced anaphylactic reaction, were partially restored with the T.g. HSP70 gene vaccination. The T.g. HSP70-induced marked production of PAF and IFN-γ from splenocytes of infected mice during the T.g. HSP70-induced anaphylactic reaction was shown to decrease after the T.g. HSP70 gene vaccination. Thus, T.g. HSP70 gene vaccine induced protective immunity against T.g. HSP70-induced PAF-mediated lethal anaphylactic reaction in T. gondii -infected mice.