The minimal SUF system is not required for Fe–S cluster biogenesis in the methanogenic archaeon Methanosarcina acetivorans
Abstract Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis sy...
Saved in:
Published in | Scientific reports Vol. 13; no. 1; pp. 15120 - 13 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group
13.09.2023
Nature Publishing Group UK Nature Portfolio |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Abstract
Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in
Methanosarcina acetivorans
, which contains two
sufCB
gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete
sufC1B1
and
sufC2B2
, respectively. Neither the dual repression of
sufC1B1
and
sufC2B2
nor the deletion of both
sufC1B1
and
sufC2B2
affected the growth of
M. acetivorans
under any conditions tested, including diazotrophy. Interestingly, deletion of only
sufC1B1
led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of
sufC2B2
acts as a suppressor mutation in the absence of
sufC1B1
. In addition, the deletion of
sufC1B1
and/or
sufC2B2
did not affect the total Fe–S cluster content in
M. acetivorans
cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in
M. acetivorans
and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. |
---|---|
AbstractList | Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in
Methanosarcina acetivorans
, which contains two
sufCB
gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete
sufC1B1
and
sufC2B2
, respectively. Neither the dual repression of
sufC1B1
and
sufC2B2
nor the deletion of both
sufC1B1
and
sufC2B2
affected the growth of
M. acetivorans
under any conditions tested, including diazotrophy. Interestingly, deletion of only
sufC1B1
led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of
sufC2B2
acts as a suppressor mutation in the absence of
sufC1B1
. In addition, the deletion of
sufC1B1
and/or
sufC2B2
did not affect the total Fe–S cluster content in
M. acetivorans
cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in
M. acetivorans
and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. Iron-sulfur (Fe-S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe-S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe-S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans, which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2, respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1. In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe-S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe-S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe-S cluster biogenesis in methanogens.Iron-sulfur (Fe-S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe-S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe-S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans, which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2, respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1. In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe-S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe-S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe-S cluster biogenesis in methanogens. Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans, which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2, respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1. In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe–S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. Abstract Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans, which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2, respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1. In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe–S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. Abstract Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans , which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2 , respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1 . In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe–S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. |
ArticleNumber | 15120 |
Author | Deere, Thomas M Saini, Jasleen Lessner, Daniel J |
Author_xml | – sequence: 1 givenname: Jasleen surname: Saini fullname: Saini, Jasleen – sequence: 2 givenname: Thomas M surname: Deere fullname: Deere, Thomas M – sequence: 3 givenname: Daniel J surname: Lessner fullname: Lessner, Daniel J |
BackLink | https://www.osti.gov/biblio/1999964$$D View this record in Osti.gov |
BookMark | eNpdkk1uFDEQhVsoiISQC7CyYMOmwb_d9gqhiIFIQSySrC3bXTPjUY-d2O4okVhwh9wwJ8EzHSFCbWw9P31Vtt_r5iDEAE3zluCPBDP5KXMilGwxZS2nHOP27kVzRDEXLWWUHvyzP2xOct7gWoIqTtSr5pD1PeZdr46aX5drQFsf_NaM6OJqgfJ9LrBFPqMQC0pwM_kEA1rGhBbw-PvhArlxqpaErI8rCJCr1QdUdhwoaxN2qnfIJLc2EAP6Mau5Cj4YZBwUfxuTCflN83JpxgwnT-txc7X4enn6vT3_-e3s9Mt56wQmpSVWqa7HA-mM4XgQYB1YXBUKVOCB2gFIz4feLqVRUjoqbN8ZyfrOESOXhB03ZzN3iGajr1O9bLrX0Xi9F2JaaZOKdyNoRYQlPVjBBeXMUomBDaJiwXCQasf6PLOuJ7uFwUEoyYzPoM9Pgl_rVbzVBIv6BURVwruZEHPxOjtfwK1dDAFc0UTV6ng1fXhqk-LNBLnorc8OxtEEiFPWVHZcKsUkrtb3_1k3cUqhPujexTGhbDc3nV0uxZwTLP-OTLDeJUrPidI1UXqfKH3H_gBKGcBo |
CitedBy_id | crossref_primary_10_3390_inorganics12040101 crossref_primary_10_1016_j_bbamcr_2024_119746 crossref_primary_10_1016_j_bbamcr_2024_119730 crossref_primary_10_1016_j_bbamcr_2024_119772 |
Cites_doi | 10.1126/science.aan0425 10.1186/s12866-020-02014-z 10.1155/2008/534081 10.1021/acs.chemrev.9b00489 10.1016/0003-2697(76)90527-3 10.1073/pnas.94.6.2626 10.1371/journal.pone.0171279 10.1128/JB.180.6.1504-1511.1998 10.1073/pnas.1618596114 10.1038/s41396-021-01028-3 10.1101/gr.223902 10.1128/JB.00117-21 10.1074/jbc.M607646200 10.1074/jbc.M110.152447 10.1128/JB.01536-09 10.1098/rstb.2013.0088 10.1128/AEM.01402-20 10.1128/JB.00146-21 10.1016/j.jinorgbio.2012.06.008 10.1074/jbc.M400278200 10.3389/fmicb.2019.02478 10.1016/0003-2697(83)90186-0 10.1128/IAI.00100-17 10.1128/AEM.03389-14 10.1073/pnas.1220225110 10.1128/aem.01033-23 10.1146/annurev.mi.49.100195.002003 10.1128/aem.59.11.3832-3839.1993 10.1126/science.aaf9284 10.1111/j.1365-2958.2004.04025.x 10.1007/s00775-017-1527-3 10.1093/genetics/152.4.1343 10.1146/annurev.biochem.74.082803.133518 10.1016/j.plasmid.2016.02.003 10.1128/jb.179.2.541-543.1997 10.1128/jb.171.2.1017-1027.1989 10.1038/s41559-022-01857-1 10.1128/JB.187.17.6137-6146.2005 10.1021/bi500488r 10.1016/S1367-5931(00)00132-0 10.1146/annurev-biochem-060614-034108 |
ContentType | Journal Article |
Copyright | The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2023. Springer Nature Limited. Springer Nature Limited 2023 |
Copyright_xml | – notice: The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2023. Springer Nature Limited. – notice: Springer Nature Limited 2023 |
CorporateAuthor | Univ. of Arkansas, Fayetteville, AR (United States) |
CorporateAuthor_xml | – name: Univ. of Arkansas, Fayetteville, AR (United States) |
DBID | AAYXX CITATION 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PIMPY PQEST PQQKQ PQUKI PRINS Q9U 7X8 OTOTI 5PM DOA |
DOI | 10.1038/s41598-023-42400-x |
DatabaseName | CrossRef ProQuest Central (Corporate) Proquest Health & Medical Complete ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) ProQuest Science Journals Biological Science Database ProQuest - Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic OSTI.GOV PubMed Central (Full Participant titles) Directory of Open Access Journals |
DatabaseTitle | CrossRef Publicly Available Content Database ProQuest Central Student ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database CrossRef |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 2045-2322 |
EndPage | 13 |
ExternalDocumentID | oai_doaj_org_article_915b17eb545243b280e3d5a98ea4e891 1999964 10_1038_s41598_023_42400_x |
GrantInformation_xml | – fundername: ; – fundername: ; grantid: DE-SC0019226 – fundername: ; grantid: MCB1817819 |
GroupedDBID | 0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD AAYXX ABDBF ABUWG ACGFS ACSMW ADBBV ADRAZ AENEX AFKRA AFPKN AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU CITATION DIK DWQXO EBD EBLON EBS ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RIG RNT RNTTT RPM SNYQT UKHRP 7XB 8FK K9. PQEST PQUKI PRINS Q9U 7X8 OTOTI 5PM |
ID | FETCH-LOGICAL-c501t-1b99670d16aa40d5ebceb06702e250d2bde174d7bf8a988c25b76a8376c1a8f13 |
IEDL.DBID | RPM |
ISSN | 2045-2322 |
IngestDate | Fri Oct 04 13:13:38 EDT 2024 Tue Sep 17 21:29:35 EDT 2024 Mon Sep 23 03:24:54 EDT 2024 Fri Aug 30 19:47:25 EDT 2024 Fri Sep 13 10:39:37 EDT 2024 Fri Aug 23 03:38:35 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
License | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c501t-1b99670d16aa40d5ebceb06702e250d2bde174d7bf8a988c25b76a8376c1a8f13 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 SC0019226 USDOE Office of Science (SC) |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500019/ |
PMID | 37704679 |
PQID | 2864401231 |
PQPubID | 2041939 |
PageCount | 13 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_915b17eb545243b280e3d5a98ea4e891 pubmedcentral_primary_oai_pubmedcentral_nih_gov_10500019 osti_scitechconnect_1999964 proquest_miscellaneous_2864899380 proquest_journals_2864401231 crossref_primary_10_1038_s41598_023_42400_x |
PublicationCentury | 2000 |
PublicationDate | 2023-09-13 |
PublicationDateYYYYMMDD | 2023-09-13 |
PublicationDate_xml | – month: 09 year: 2023 text: 2023-09-13 day: 13 |
PublicationDecade | 2020 |
PublicationPlace | London |
PublicationPlace_xml | – name: London – name: United States |
PublicationTitle | Scientific reports |
PublicationYear | 2023 |
Publisher | Nature Publishing Group Nature Publishing Group UK Nature Portfolio |
Publisher_xml | – name: Nature Publishing Group – name: Nature Publishing Group UK – name: Nature Portfolio |
References | C Johnson (42400_CR21) 2021; 203 FL Sousa (42400_CR10) 2013; 368 AE Dhamad (42400_CR22) 2020; 86 G Huet (42400_CR6) 2005; 187 PS Kessler (42400_CR35) 1997; 179 KR Sowers (42400_CR37) 1993; 59 WW Metcalf (42400_CR38) 1997; 94 AG Albrecht (42400_CR5) 2010; 192 JW Peters (42400_CR3) 1995; 49 Y Liu (42400_CR11) 2010; 285 AM Guss (42400_CR42) 2008; 2 H Beinert (42400_CR39) 1983; 131 DC Johnson (42400_CR1) 2005; 74 J Perard (42400_CR15) 2018; 23 S Jang (42400_CR8) 2007; 282 MR Jacobson (42400_CR2) 1989; 171 MT Shea (42400_CR40) 2016; 84–85 PS Kessler (42400_CR33) 1998; 180 DD Nayak (42400_CR24) 2017; 114 T Wagner (42400_CR13) 2017; 357 JE Galagan (42400_CR27) 2002; 12 PC Dos Santos (42400_CR32) 2004; 279 PS Kessler (42400_CR34) 1999; 152 D Payne (42400_CR25) 2021; 203 AD Tsaousis (42400_CR7) 2019; 10 Y Hu (42400_CR30) 2016; 85 C Andreini (42400_CR9) 2017; 12 FW Outten (42400_CR29) 2004; 52 PS Garcia (42400_CR18) 2022; 6 TM Deere (42400_CR19) 2020; 20 K Lang (42400_CR36) 2015; 81 M Chanderban (42400_CR23) 2023 D Payne (42400_CR26) 2021; 15 B Roche (42400_CR4) 2013; 1827 HK Chahal (42400_CR16) 2012; 116 ES Boyd (42400_CR17) 2014; 53 T Wagner (42400_CR12) 2016; 354 S Buren (42400_CR31) 2020; 120 CA Roberts (42400_CR28) 2017; 85 MM Bradford (42400_CR41) 1976; 72 F Sarmiento (42400_CR20) 2013; 110 DC Rees (42400_CR14) 2000; 4 |
References_xml | – volume: 357 start-page: 699 year: 2017 ident: 42400_CR13 publication-title: Science doi: 10.1126/science.aan0425 contributor: fullname: T Wagner – volume: 20 start-page: 323 year: 2020 ident: 42400_CR19 publication-title: BMC Microbiol. doi: 10.1186/s12866-020-02014-z contributor: fullname: TM Deere – volume: 2 start-page: 193 year: 2008 ident: 42400_CR42 publication-title: Archaea doi: 10.1155/2008/534081 contributor: fullname: AM Guss – volume: 120 start-page: 4921 year: 2020 ident: 42400_CR31 publication-title: Chem. Rev. doi: 10.1021/acs.chemrev.9b00489 contributor: fullname: S Buren – volume: 72 start-page: 248 year: 1976 ident: 42400_CR41 publication-title: Anal Biochem. doi: 10.1016/0003-2697(76)90527-3 contributor: fullname: MM Bradford – volume: 94 start-page: 2626 year: 1997 ident: 42400_CR38 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.94.6.2626 contributor: fullname: WW Metcalf – volume: 1827 start-page: 455 year: 2013 ident: 42400_CR4 publication-title: Biochem. Biophys. Acta contributor: fullname: B Roche – volume: 12 year: 2017 ident: 42400_CR9 publication-title: PLoS ONE doi: 10.1371/journal.pone.0171279 contributor: fullname: C Andreini – volume: 180 start-page: 1504 year: 1998 ident: 42400_CR33 publication-title: J. Bacteriol. doi: 10.1128/JB.180.6.1504-1511.1998 contributor: fullname: PS Kessler – volume: 114 start-page: 2976 year: 2017 ident: 42400_CR24 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1618596114 contributor: fullname: DD Nayak – volume: 15 start-page: 3498 year: 2021 ident: 42400_CR26 publication-title: ISME J. doi: 10.1038/s41396-021-01028-3 contributor: fullname: D Payne – volume: 12 start-page: 532 year: 2002 ident: 42400_CR27 publication-title: Genome Res. doi: 10.1101/gr.223902 contributor: fullname: JE Galagan – volume: 203 year: 2021 ident: 42400_CR21 publication-title: J. Bacteriol. doi: 10.1128/JB.00117-21 contributor: fullname: C Johnson – volume: 282 start-page: 929 year: 2007 ident: 42400_CR8 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M607646200 contributor: fullname: S Jang – volume: 285 start-page: 31923 year: 2010 ident: 42400_CR11 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M110.152447 contributor: fullname: Y Liu – volume: 192 start-page: 1643 year: 2010 ident: 42400_CR5 publication-title: J. Bacteriol. doi: 10.1128/JB.01536-09 contributor: fullname: AG Albrecht – volume: 368 start-page: 20130088 year: 2013 ident: 42400_CR10 publication-title: Philos. Trans. R. Soc. Lond. B Biol. Sci. doi: 10.1098/rstb.2013.0088 contributor: fullname: FL Sousa – volume: 86 start-page: e01402-20 year: 2020 ident: 42400_CR22 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.01402-20 contributor: fullname: AE Dhamad – volume: 203 year: 2021 ident: 42400_CR25 publication-title: J. Bacteriol. doi: 10.1128/JB.00146-21 contributor: fullname: D Payne – volume: 116 start-page: 126 year: 2012 ident: 42400_CR16 publication-title: J. Inorg. Biochem. doi: 10.1016/j.jinorgbio.2012.06.008 contributor: fullname: HK Chahal – volume: 279 start-page: 19705 year: 2004 ident: 42400_CR32 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M400278200 contributor: fullname: PC Dos Santos – volume: 10 start-page: 2478 year: 2019 ident: 42400_CR7 publication-title: Front. Microbiol. doi: 10.3389/fmicb.2019.02478 contributor: fullname: AD Tsaousis – volume: 131 start-page: 373 year: 1983 ident: 42400_CR39 publication-title: Anal. Biochem. doi: 10.1016/0003-2697(83)90186-0 contributor: fullname: H Beinert – volume: 85 start-page: 10 year: 2017 ident: 42400_CR28 publication-title: Infect. Immun. doi: 10.1128/IAI.00100-17 contributor: fullname: CA Roberts – volume: 81 start-page: 1338 year: 2015 ident: 42400_CR36 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.03389-14 contributor: fullname: K Lang – volume: 110 start-page: 4726 year: 2013 ident: 42400_CR20 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1220225110 contributor: fullname: F Sarmiento – year: 2023 ident: 42400_CR23 publication-title: Appl. Environ. Microbiol. doi: 10.1128/aem.01033-23 contributor: fullname: M Chanderban – volume: 49 start-page: 335 year: 1995 ident: 42400_CR3 publication-title: Annu. Rev. Microbiol. doi: 10.1146/annurev.mi.49.100195.002003 contributor: fullname: JW Peters – volume: 59 start-page: 3832 year: 1993 ident: 42400_CR37 publication-title: Appl. Environ. Microbiol. doi: 10.1128/aem.59.11.3832-3839.1993 contributor: fullname: KR Sowers – volume: 354 start-page: 114 year: 2016 ident: 42400_CR12 publication-title: Science doi: 10.1126/science.aaf9284 contributor: fullname: T Wagner – volume: 52 start-page: 861 year: 2004 ident: 42400_CR29 publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2004.04025.x contributor: fullname: FW Outten – volume: 23 start-page: 581 year: 2018 ident: 42400_CR15 publication-title: J. Biol. Inorg. Chem. doi: 10.1007/s00775-017-1527-3 contributor: fullname: J Perard – volume: 152 start-page: 1343 year: 1999 ident: 42400_CR34 publication-title: Genetics doi: 10.1093/genetics/152.4.1343 contributor: fullname: PS Kessler – volume: 74 start-page: 247 year: 2005 ident: 42400_CR1 publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev.biochem.74.082803.133518 contributor: fullname: DC Johnson – volume: 84–85 start-page: 27 year: 2016 ident: 42400_CR40 publication-title: Plasmid doi: 10.1016/j.plasmid.2016.02.003 contributor: fullname: MT Shea – volume: 179 start-page: 541 year: 1997 ident: 42400_CR35 publication-title: J. Bacteriol. doi: 10.1128/jb.179.2.541-543.1997 contributor: fullname: PS Kessler – volume: 171 start-page: 1017 year: 1989 ident: 42400_CR2 publication-title: J. Bacteriol. doi: 10.1128/jb.171.2.1017-1027.1989 contributor: fullname: MR Jacobson – volume: 6 start-page: 1564 year: 2022 ident: 42400_CR18 publication-title: Nat. Ecol. Evol. doi: 10.1038/s41559-022-01857-1 contributor: fullname: PS Garcia – volume: 187 start-page: 6137 year: 2005 ident: 42400_CR6 publication-title: J. Bacteriol. doi: 10.1128/JB.187.17.6137-6146.2005 contributor: fullname: G Huet – volume: 53 start-page: 5834 year: 2014 ident: 42400_CR17 publication-title: Biochemistry doi: 10.1021/bi500488r contributor: fullname: ES Boyd – volume: 4 start-page: 559 year: 2000 ident: 42400_CR14 publication-title: Curr. Opin. Chem. Biol. doi: 10.1016/S1367-5931(00)00132-0 contributor: fullname: DC Rees – volume: 85 start-page: 455 year: 2016 ident: 42400_CR30 publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-060614-034108 contributor: fullname: Y Hu |
SSID | ssj0000529419 |
Score | 2.4627843 |
Snippet | Abstract
Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy).... Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy).... Iron-sulfur (Fe-S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy).... Abstract Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy).... |
SourceID | doaj pubmedcentral osti proquest crossref |
SourceType | Open Website Open Access Repository Aggregation Database |
StartPage | 15120 |
SubjectTerms | Archaea Biochemistry Biosynthesis CRISPR Evolution Gene clusters Gene deletion Genetic suppression Growth conditions Metals Methanogenesis Methanogenic bacteria Methanosarcina acetivorans Microbial genetics Microbiology Nitrogen fixation Phenotypes Science & Technology - Other Topics Sulfur |
SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Ni9UwFA0yILgRxw-sM0oEdxKmSZM2XY7iYxDGzfhgdiEf92lBW5nXBzPgwv_gP_SXeG_TGV5XblwVkrS0OTfJuc3NuYy9QZaKpqOk8LE0QsvkhU0GBOIRYWMbVSZyFM8_1Wdr_fHSXO6l-qKYsCwPnDvupJUmyAYC5cLWVVC2hCoZ31rwGmybHR9p9pyprOqtWi3b-ZRMWdmTLa5UdJpMVUJT3KS4XqxEk2A_XgYcWAuyuQyV3Ft7Vo_Yw5k08tP8sofsHvSP2f2cRvLmCfuJWHPSCPmOjS7WK57lmXm35f0w8iugaF9IHPkpX8GfX78vePy2I4UEHrrhC8122LTr-UjPAfqZTqVd5JOSEgw9P8-lW8o81HvuI-A0OdA695StVx8-vz8Tc1YFEU0pRyEDujgNQlB7r0vEJUQIdFpHAdKhpEIC9FJSEzYWe9lGZUJTe_Rj6yi93cjqGTvohx6eMw4ylsoDbLRvdZOC10E3bVJQK-VTYwr29raH3Y8snuGmTe_KuoyHQzzchIe7Ltg7AuGuJQlfTwVoDm42B_cvcyjYEUHokD-QCG6kaKE4OhJbaGtdsONbZN08VrdOWeSERC3x5td31TjKaOvE9zDschv0TCtbFswuLGLxusuavvs66XUjhZ2o9Iv_8YFH7AFlvKeQFVkds4PxagcvkReN4dU0BP4CCjoM7Q priority: 102 providerName: Directory of Open Access Journals – databaseName: Proquest Health & Medical Complete dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Ni9RAEG3WFcGLrF8Yd5UWvEmYdKeT7pzEFYdBWC_rwNya_qhZA5rszmRgBQ_-B_-hv8SqJDOai6dAp0lCXnXXq-7qV4y9RpaKpiNF6kJWpEpEl5pYQIp4BFgbLbNIgeLFp3KxVB9XxeqILfZnYSitcj8n9hN1bAOtkc-kQc9NBEDMnKdVgNDN3l7fpFQ_ivZZx2Iad9hdIZFWoGXrlT6sttB-lhLVeGomy81si56LTpfJPFWUR5neTjxTL-CPlxYH2oR8TlMn__FF8xP2YCSR_N2A-kN2BM0jdm8oK_n9MfuB2HPSDPmGnS6Xcz7INfN6y5u24xug7F-IHPkqn8Pvn78uefi6I8UE7uv2imY_7Fo3vKPnAC2uU2sdeK-sBG3DL4bWLVUiahx3AXDabMnvPWHL-YfP7xfpWGUhDUUmulR4DHk0QlI6pzLEyQfwdHpHAtKjKH0EjFqi9mvjKmOCLLwuHca1ZRDOrEX-lB03bQPPGAcRMukA1spVSkfvlFe6ihJKKV3URcLe7P-wvR7ENGy_CZ4bO-BhEQ_b42FvE3ZOIBx6khB239Buruw4rmwlCi80eCqVrnIvTQZ5LPBDwSkwlUjYKUFokU-QKG6g7KHQWRJfqEqVsLM9snYcu1v719IS9upwG0cdbaW4Btrd0Acj1dxkCTMTi5h87vROU3_p9buR0vbU-vn_337K7lNte0pOEfkZO-42O3iBDKjzL3vj_gN_rgit priority: 102 providerName: ProQuest – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Na9wwEBUhodBL6Sd1kxYVeitqLVm25UMpSekSCttLupCb0MdsakjsdtcLCeSQ_9B_2F-SGdsbasilJ4MsC6E3kt5YozeMvUOWiqajpHAhzYWW0QkTcxCIR4ClKVUayVGcfy-OF_rbaX66w7bpjsYBXN_r2lE-qcXq_MPl76vPOOE_DVfGzcc1bkJ0UUxlQlNIpEBOuad0psni5yPdH7S-VaX7XB8kwi6QTKjxHs39zUz2ql7SHx8tTr0JHZ0GU_6zO80es0cjreSHgx08YTvQPGUPhkSTV8_YNVoDJxWRC6x0spjxQcCZ12vetB1fAcUDQ-TIYPkM_t78OeHhfEMaCtzX7Rmth1i1bnhH7QD9bqfSOvBeawnahs-H0jXlJmocdwFwIW1pJ3zOFrOvP74cizHvggh5KjshPTpBJYJUOKdTRM4H8HSfRwESpqh8BPRjYumXxlXGBJX7snDo6RZBOrOU2Qu227QNvGQcZEiVA1hqV-kyeqe9LquooFDKxTJP2PvtCNtfg7yG7Y_FM2MHPCziYXs87GXCjgiEu5okjd0XtKszO840W8ncyxI8JU_XmVcmhSzm2FFwGkwlE7ZPEFpkGCSTGyieKHSW5BiqQifsYIus3RqjVQZZI5FP_Pjt3Wuch3S44hpoN0Md9F0zkybMTCxi0t3pm6b-2St6I8ntyfar_xqOffZQkbVSQovsgO12qw28RorU-Te93d8CBsYOWQ priority: 102 providerName: Scholars Portal |
Title | The minimal SUF system is not required for Fe–S cluster biogenesis in the methanogenic archaeon Methanosarcina acetivorans |
URI | https://www.proquest.com/docview/2864401231/abstract/ https://www.proquest.com/docview/2864899380/abstract/ https://www.osti.gov/biblio/1999964 https://pubmed.ncbi.nlm.nih.gov/PMC10500019 https://doaj.org/article/915b17eb545243b280e3d5a98ea4e891 |
Volume | 13 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Na9tAEF2SlEIvpZ_USWq20FtRrP2QdnVsQkwoOISmBt-W3dU4FcRSsGVooYf-h_7D_pLOSHawr71IsFqJRW9G-2Y1-4axj8hS0XSkSHxMs0SL0ie2zCBBPCLMrZFpSYHi5Dq_muovs2x2wPLtXpguaT-G6qy-X5zV1fcut_JhEUfbPLHRzeQCOUHHTUaH7NAotROj94restCi2OyQSZUdrXCWop1kUiWaciYTUp9WxmBsSClcOxNSp9uPpwb9a49z7mdM7kxB4xfs-YY78s_9GF-yA6hfsad9Ncmfr9kvhJyTVMgCO91Ox7xXaebVitdNy5dASb9QcqSpfAx_f_-55fF-TUIJPFTNHX30sGtV85aeA7SmTq1V5J2gEjQ1n_StKypAVHvuI-DXsqHp7g2bji-_XVwlm-IKScxS0SYiYKRjEInce50iPCFCoE07EpAVlTKUgMFKacLc-sLaKLNgco_hbB6Ft3Oh3rKjuqnhHeMgYio9wFz7QpsyeB20KUoJuZS-NNmAfdq-YffQa2i47t-3sq6HxiE0roPG_RiwcwLhsSfpX3cNzfLObazAFSILwkCgCulaBWlTUGWGAwWvwRZiwE4IQoc0grRwIyUNxdaR5kKR6wE73SLrNi67ctIiNSSGiTd_eLyMzkZ_UHwNzbrvgwGqsumA2T2L2Bvu_hW04k62e2u1x_9_6wl7RuXuKV9FqFN21C7X8B5JURuG6AkzM2RPzi-vb74Ou6UFPE60HXbe8Q9V3RKf |
link.rule.ids | 230,315,733,786,790,870,891,2115,2236,12083,21416,24346,27957,27958,31754,31755,33779,33780,43345,43840,53827,53829,74102,74659 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwELVgKwQXxKcILWAkbihq7DiJc0IUdbVAd4VoV-rN8sdsuxIkZTcrFYkD_4F_yC9hJsku5MIpkm0lVp7teWOP3zD2ClkqDh0pYuuTLFYi2FiHDGLEw8NCFzIJ5ChOZ_lkrj6cZ-f9htu6D6vcrontQh1qT3vkh1Kj5SYCIN5cfYspaxSdrvYpNG6yPZWiqzJie0fHs0-fd7ssdI6lRNnflklSfbhGi0W3ymQaK4qfjK8HFqkV7sdHjRNsQDqHIZP_2KDxPXa3J4_8bYf2fXYDqgfsVpdO8vtD9gMx56QV8hUbnc7HvJNp5ss1r-qGr4CifiFw5Kl8DL9__jrl_suGlBK4W9YXtOph02XFG3oP0KY6lS49bxWVoK74tCtdUwaiynLrAZfLmuzdIzYfH5-9m8R9doXYZ4loYuHQ1SkQitxalSA-zoOjWzsSkBYF6QKgtxIKt9C21NrLzBW5RX8298LqhUgfs1FVV_CEcRA-kRZgoWypiuCscqoog4RcShuKLGKvt3_YXHUiGqY9_E616fAwiIdp8TDXETsiEHYtSQC7LahXF6afT6YUmRMFOEqRrlIndQJpyLCjYBXoUkRsnyA0yCNIDNdT1JBvDIkulLmK2MEWWdPP2bX5O8Ii9nJXjbONjlBsBfWma4MeaqqTiOnBiBh0d1hTLS9b3W6ksi2lfvr_r79gtydn0xNz8n72cZ_dofz2FKAi0gM2alYbeIYsqHHP-6H-BwC1CUY |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagFYgL4qmGFjASNxRt7DiJc0IUuiqPrirKSr1ZfkzKSpCU3axUJA78B_4hv4SZJLuQC6dIjpVE-caeb-zxN4w9R5aKpiNFbH2SxUoEG-uQQYx4eKh0IZNAgeLJLD-eq3fn2fmQ_7Qa0io3c2I3UYfG0xr5RGr03EQAxKQa0iJO30xfXn6LqYIU7bQO5TSus91C5Rla-O7h0ez043bFhfa0lCiHkzNJqicr9F50wkymsaJcyvhq5J06EX-8NDjYRgR0nD75jz-a3mG3ByLJX_XI32XXoL7HbvSlJb_fZz8Qf066IV-x09l8ynvJZr5Y8bpp-RIoAxgCR87Kp_D7568z7r-sSTWBu0VzQTMgdl3UvKXnAC2wU-vC805dCZqan_StK6pGVFtuPeDU2ZDve8Dm06NPr4_jodJC7LNEtLFwGPYUCEturUoQK-fB0QkeCUiRgnQBMHIJhau0LbX2MnNFbjG2zb2wuhLpQ7ZTNzXsMQ7CJ9ICVMqWqgjOKqeKMkjIpbShyCL2YvOHzWUvqGG6jfBUmx4Pg3iYDg9zFbFDAmHbk8Swu4ZmeWGGsWVKkTlRgKNy6Sp1UieQhgw_FKwCXYqI7ROEBjkFCeN6yiDyrSEBhjJXETvYIGuG8bsyf60tYs-2t3Hk0XaKraFZ930wWk11EjE9sojR547v1IvPnYY30tqOXj_6_9ufspto5ebD29n7fXaLSt1TropID9hOu1zDYyRErXsyWPofWqsNgw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+minimal+SUF+system+is+not+required+for+Fe%E2%80%93S+cluster+biogenesis+in+the+methanogenic+archaeon+Methanosarcina+acetivorans&rft.jtitle=Scientific+reports&rft.au=Saini%2C+Jasleen&rft.au=Deere%2C+Thomas+M.&rft.au=Lessner%2C+Daniel+J.&rft.date=2023-09-13&rft.issn=2045-2322&rft.eissn=2045-2322&rft.volume=13&rft.issue=1&rft_id=info:doi/10.1038%2Fs41598-023-42400-x&rft.externalDBID=n%2Fa&rft.externalDocID=10_1038_s41598_023_42400_x |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon |