The minimal SUF system is not required for Fe–S cluster biogenesis in the methanogenic archaeon Methanosarcina acetivorans
Abstract Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis sy...
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Published in | Scientific reports Vol. 13; no. 1; pp. 15120 - 13 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group
13.09.2023
Nature Publishing Group UK Nature Portfolio |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract
Iron–sulfur (Fe–S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe–S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe–S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in
Methanosarcina acetivorans
, which contains two
sufCB
gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete
sufC1B1
and
sufC2B2
, respectively. Neither the dual repression of
sufC1B1
and
sufC2B2
nor the deletion of both
sufC1B1
and
sufC2B2
affected the growth of
M. acetivorans
under any conditions tested, including diazotrophy. Interestingly, deletion of only
sufC1B1
led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of
sufC2B2
acts as a suppressor mutation in the absence of
sufC1B1
. In addition, the deletion of
sufC1B1
and/or
sufC2B2
did not affect the total Fe–S cluster content in
M. acetivorans
cells. Overall, these results reveal that the minimal SUF system is not required for Fe–S cluster biogenesis in
M. acetivorans
and challenge the universal role of SufBC in Fe–S cluster biogenesis in methanogens. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 SC0019226 USDOE Office of Science (SC) |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-023-42400-x |