Effect of heme oxygenase-1 induction by octreotide on TNBS-induced colitis

• Published in: Journal of gastroenterology and hepatology Vol. 22; no. 11; pp. 1852 - 1858
• Main Authors: Erbil, Yeşim, Giriş, Murat, Abbasoğlu, Semra Doğru, Barbaros, Umut, Yanık, Burcu Tulumoğlu, Necefli, Ahmet, Olgaç, Vakur, Toker, Gülçin Aykaç
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.11.2007; Blackwell Science
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants - pharmacology; Antioxidants - therapeutic use; Biological and medical sciences; Blotting, Western; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - enzymology; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Colitis, Ulcerative - prevention & control; Colon - drug effects; Colon - enzymology; Colon - metabolism; Colon - pathology; Disease Models, Animal; Enzyme Induction - drug effects; Gastroenterology. Liver. Pancreas. Abdomen; Gastrointestinal Agents - pharmacology; Gastrointestinal Agents - therapeutic use; Glutathione - metabolism; Heme Oxygenase (Decyclizing) - biosynthesis; heme oxygenase-1; Male; Malondialdehyde - metabolism; Medical sciences; NF-kappa B - metabolism; octreotide; Octreotide - pharmacology; Octreotide - therapeutic use; Other diseases. Semiology; Oxidative Stress - drug effects; Rats; Rats, Wistar; Severity of Illness Index; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Time Factors; TNBS-induced colitis; Trinitrobenzenesulfonic Acid; Immunohistochemistry; Chronic disease; Mucosa; Octreotide; heme oxygenase-1; Inflammatory disease; Anatomic pathology; Histopathology; Treatment; Animal; Gastroenterology; Rectum; Digestive diseases; Intestinal disease; Colitis; Colon; TNBS-induced colitis; Ulcerative colitis
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1111/j.1440-1746.2007.04838.x

Background and Aim:  Ulcerative colitis is a chronic inflammatory disease of the colon and rectum. Although the precise etiology of ulcerative colitis remains unknown, it is believed to involve an abnormal host response to endogenous or environmental antigens, genetic factors, and oxidative damage. The aim of the present study was to investigate whether heme oxygenase‐1 (HO‐1) induction by octreotide could protect against oxidative and inflammatory damage from induced colitis. Methods:  Rats received octreotide 50 µg/kg per day intraperitoneally for 5 days before 2,4,6 trinitrobenzene sulfonic acid (TNBS) solution administration and for 15 days following TNBS solution administration. Rats were killed on day 21, and colonic malondialdehyde (MDA) levels, glutathione (GSH) levels and HO‐1 expression were measured. Nuclear factor (NF)‐κB and HO‐1 expression was evaluated by immunohistochemical examination of the colonic tissue. Results:  Rats with TNBS‐induced colitis had significantly increased colonic MDA levels and HO‐1 expression in comparison to the control group. Octreotide treatment was associated with increased HO‐1 expression and GSH levels, but decreased MDA levels. Histopathological examination revealed that the intestinal mucosal structure was preserved in the octreotide‐treated group. In addition, treatment with octreotide significantly increased HO‐1 expression and decreased NF‐κB expression by immunohistochemistry when compared to the TNBS‐induced colitis group. Conclusion:  Octreotide appears to have protective effects against colonic damage in TNBS‐induced colitis. This protective effect is, in part, mediated by modification of the inflammatory response and the induction of HO‐1 expression.