Insulin-like Effects of Vanadate on Rat Liver 6-Phosphofructo-2-Kinase/Fructose-2, 6-Bisphosphatase mRNA and Protein Inductions in Diabetic Rats

• Published in: Endocrine Journal Vol. 41; no. 1; pp. 75 - 82
• Main Authors: INOUE, HIROSHI, KAKU, KOHEI, MATSUTANI, AKIRA, TAO, TSUYOSHI, AYAME, HIDEO, KANEKO, TOSHIO
• Format: Journal Article
• Language: English
• Published: Tokyo The Japan Endocrine Society 1994; Japan Endocrine Society
• Subjects: 6PF-2-K/Fru-2,6-P2ase mRNA; Animals; Biological and medical sciences; Blood Glucose - metabolism; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - enzymology; Diabetes Mellitus, Experimental - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Insulin; Insulin - blood; Insulin - pharmacology; Liver - drug effects; Liver - enzymology; Medical sciences; Phosphofructokinase-2; Phosphoric Monoester Hydrolases - genetics; Phosphotransferases (Alcohol Group Acceptor) - genetics; Protein Biosynthesis; Rats; RNA, Messenger - drug effects; STZ-diabetic rat; Vanadate; Vanadates - pharmacology; Endocrinopathy; Rat; Enzyme; Transferases; Diabetes mellitus; Liver; Rodentia; Phosphoric monoester hydrolases; Fructose-2,6-bisphosphate 2-phosphatase; Esterases; Insulin; Trace element; Vertebrata; Mammalia; Messenger RNA; Animal; Vanadates; Hydrolases; 6-Phosphofructo-2-kinase
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.41.75

Effects of vanadate on liver 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (6PF-2-K/F-2, 6-P2ase) mRNA and protein inductions were examined in streptozotocin (STZ)-induced diabetic rats. In diabetic rats at one week after STZ (60mg/kg body weight), the liver 6PF-2-K activity was decreased to 22% of the control. The enzyme protein was also decreased to 31% of the control, but the reduction in mRNA was not significant. Treatment of diabetes with vanadate (10mg/kg BW, i.v., every 8h), as well as insulin (10u/kg BW, s.c., every 8h), increased the 6PF-2-K activity and theenzyme protein content, though it was not completely restored to the control level. 68% of the control was the figure for enzyme activity and 65% of the control for protein content after 24-h of treatment. On the other hand, vanadate, like insulin, increased enzyme mRNA content to a higher level than the control (140% of the control). The present results indicate that vanadate, like insulin, modulates the liver 6PF-2-K/Fru-2, 6-P2ase gene expression, and stimulated protein induction contribu tes to the regulation of its enzyme activity, resulting in amelioration of the deranged carbohydrate metabolism in the diabetic state.