Enzymatically induced mineralization of platelet-rich fibrin

• Published in: Journal of biomedical materials research. Part A Vol. 100A; no. 5; pp. 1335 - 1346
• Main Authors: Douglas, Timothy E. L., Gassling, Volker, Declercq, Heidi A., Purcz, Nicolai, Pamula, Elzbieta, Haugen, Håvard J., Chasan, Safak, de Mulder, Eric L. W., Jansen, John A., Leeuwenburgh, Sander C. G.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2012; Wiley-Blackwell
• Subjects: alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Blood Platelets - metabolism; Blood. Blood coagulation. Reticuloendothelial system; bone tissue engineering; Calcification, Physiologic; Cattle; Cell Death; Cell Line, Tumor; Cell physiology; Fibrin - metabolism; Freeze Drying; Fundamental and applied biological sciences. Psychology; Humans; hydrogel; Medical sciences; Microscopy, Electron, Scanning; Microscopy, Fluorescence; mineralization; Mineralization, calcification; Molecular and cellular biology; Pharmacology. Drug treatments; platelet-rich fibrin; Spectrometry, X-Ray Emission; Spectroscopy, Fourier Transform Infrared; Staining and Labeling; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thermogravimetry; Alkaline phosphatase; Enzyme; Tissue engineering; bone tissue engineering; Phosphoric monoester hydrolases; Esterases; Fibrin glue; platelet-rich fibrin; Osteoarticular system; Mineralization; Hydrolases; Biomaterial; Hydrogel; Bone; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.34073

Membranes of the autologous blood‐derived biomaterial platelet‐rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, EDS, FTIR, and von Kossa staining. The mass percentage attributable to CaP was quantified by lyophilization and measurement of the remaining mass fraction as well as by TGA. Cytocompatibility tests (LDH, MTT, and WST) with SAOS‐2 cells showed that mineralized PRF did not release substances detrimental to cell vitality. Live/dead staining and SEM showed that mineralized PRF was colonized by cells. The results show that hydrogel biomaterials such as PRF can be mineralized through functionalization with ALP. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.