Targeted Proteomics to Identify Cadmium-Induced Protein Modifications in Glomus mosseae-Inoculated Pea Roots

• Published in: The New phytologist Vol. 157; no. 3; pp. 555 - 567
• Main Authors: Repetto, Ombretta, Bestel-Corre, Gwénäelle, Dumas-Gaudot, Eliane, Berta, Graziella, Gianinazzi-Pearson, Vivienne, Gianinazzi, Silvio
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science 01.03.2003; Blackwell Science Ltd; Blackwell; Wiley
• Subjects: Agronomy. Soil science and plant productions; Annexins; Biological and medical sciences; Cadmium; cadmium stress; Economic plant physiology; Electrophoresis; Fundamental and applied biological sciences. Psychology; Gels; Genotypes; Glomus mosseae; Life Sciences; liquid chromatography–tandem mass spectrometry (LC‐MS; Microbial colonization; Mosses; MS; one‐ and two‐dimensional gel electrophoresis (1‐DE and 2‐DE) analyses; Parasitism and symbiosis; Peas; Phytopathology and phytopharmacy; Pisum sativum; plant biomass; Plant physiology and development; Plant roots; Plants; protein profiles; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Vegetal Biology; plant biomass; Phycomycetes; Glomus mosseae; cadmium stress; Biomass; Fungi; one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses; Dicotyledones; Angiospermae; Development; liquid chromatography-tandem mass spectrometry (LC-MS/MS); Thallophyta; Symbiosis; Cadmium; Symbionte; Tolerance; Heavy metal; Grain legume; Endomycorrhiza; Leguminosae; Protein synthesis; protein profiles; Pisum sativum; Mycorrhiza; Proteomics; Spermatophyta; liquid chromatography-tandem mass spectrometry (LC-MS; MS
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.2003.00682.x

• Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process. • The effect of Cd (100 mg Cd kg-1 substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses. • Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography-tandem mass spectrometry, newly induced and upregulated or downregulated by Cd. • The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants.