Anoxygenic phototrophic arsenite oxidation by a Rhodobacter strain

Microbially mediated arsenic redox transformations are key for arsenic speciation and mobility in rice paddies. Whereas anaerobic anoxygenic photosynthesis coupled to arsenite (As(III)) oxidation has been widely examined in arsenic‐replete ecosystems, it remains unknown whether this light‐dependent...

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Published inEnvironmental microbiology Vol. 25; no. 8; pp. 1538 - 1548
Main Authors Wu, Yi‐Fei, Chen, Jian, Xie, Wan‐Ying, Peng, Chao, Tang, Shi‐Tong, Rosen, Barry P., Kappler, Andreas, Zhang, Jun, Zhao, Fang‐Jie
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.08.2023
Wiley Subscription Services, Inc
Subjects
Anoxia
Arsenates
Arsenic
Arsenite
Arsenites
Bacteria
Biogeochemistry
carbon
Carbon sources
Ecosystem
Gene sequencing
Genomes
malates
Microbiological strains
microbiology
multigene family
oxidants
Oxidase
Oxidation
Oxidation-Reduction
Oxidizing agents
Oxidoreductases
paddies
paddy soils
Photosynthesis
Rhodobacter
Rhodobacter - genetics
Rhodobacter capsulatus
rice
Rice fields
Soil
Soil contamination
Soil pollution
Soils
Speciation
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Summary:Microbially mediated arsenic redox transformations are key for arsenic speciation and mobility in rice paddies. Whereas anaerobic anoxygenic photosynthesis coupled to arsenite (As(III)) oxidation has been widely examined in arsenic‐replete ecosystems, it remains unknown whether this light‐dependent process exists in paddy soils. Here, we isolated a phototrophic purple bacteria, Rhodobacter strain CZR27, from an arsenic‐contaminated paddy soil and demonstrated its capacity to oxidize As(III) to arsenate (As(V)) using malate as a carbon source photosynthetically. Genome sequencing revealed an As(III)‐oxidizing gene cluster (aioXSRBA) encoding an As(III) oxidase. Functional analyses showed that As(III) oxidation under anoxic phototrophic conditions correlated with transcription of the large subunit of the As(III) oxidase aioA gene. Furthermore, the non‐As(III) oxidizer Rhodobacter capsulatus SB1003 heterologously expressing aioBA from strain CZR27 was able to oxidize As(III), indicating that aioBA was responsible for the observed As(III) oxidation in strain CZR27. Our study provides evidence for the presence of anaerobic photosynthesis‐coupled As(III) oxidation in paddy soils, highlighting the importance of light‐dependent, microbe‐mediated arsenic redox changes in paddy arsenic biogeochemistry.
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AUTHOR CONTRIBUTIONS
Yi-Fei Wu: Conceptualization (equal); data curation (equal); writing – original draft (equal). Jian Chen: Methodology (equal). Wan-Ying Xie: Data curation (equal); writing – review and editing (equal). Chao Peng: Methodology (equal); resources (equal). Shi-Tong Tang: Investigation (equal); methodology (equal). Barry P. Rosen: Conceptualization (equal); data curation (equal); funding acquisition (equal); writing – review and editing (equal). Andreas Kappler: Resources (equal); writing – review and editing (equal). Jun Zhang: Methodology (equal); resources (equal). Fangjie Zhao: Resources (equal); supervision (equal); validation (equal).
ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/1462-2920.16380