Comparative floral development of Mir-grown and ethylene-treated, earth-grown Super Dwarf wheat

• Published in: Journal of plant physiology Vol. 158; no. 8; pp. 1051 - 1060
• Main Authors: Campbell, William F., Salisbury, Frank B., Bugbee, Bruce, Klassen, Steven, Naegle, Erin, Strickland, Darren T., Bingham, Gail E., Levinskikh, Margarita, Iljina, Galena M., Veselova, Tatjana D., Sytchev, Vladimir N., Podolsky, Igor, McManus, William R., Bubenheim, David L., Stieber, Joseph, Jahns, Gary
• Format: Journal Article
• Language: English
• Published: Ames Research Center Elsevier GmbH 2001; Urban and Fischer Verlag
• Subjects: Carbon Dioxide - pharmacology; ethylene; Ethylenes - pharmacology; Germination - drug effects; Life Sciences (General); microgravity; Microscopy, Confocal; Microscopy, Electron, Scanning; Mir; Plant Growth Regulators - pharmacology; pollen; Pollen - drug effects; Pollen - growth & development; seed set; Seeds - drug effects; Seeds - growth & development; Space Flight; Space life sciences; sterility; Super Dwarf; Svet; Triticum - drug effects; Triticum - growth & development; Triticum aestivum; Weightlessness; wheat; microgravity; sterility; seed set; pollen; Svet; Mir; Super Dwarf; wheat; ethylene; Triticum aestivum; NASA Experiment Number 284093 1/2; NASA Discipline Life Support Systems; Non-NASA Center
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1078/S0176-1617(04)70129-7

To study plant growth in microgravity, we grew Super Dwarf wheat ( Triticum aestivum L.) in the Svet growth chamber onboard the orbiting Russian space station, Mir, and in identical ground control units at the Institute of BioMedical Problems in Moscow, Russia. Seedling emergence was 56 percnt; and 73 percnt; in the two root-module compartments on Mir and 75 percnt; and 90 percnt; on earth. Growth was vigorous (produced ca. 1 kg dry mass), and individual plants produced 5 to 8 tillers on Mir compared with 3 to 5 on earth-grown controls. Upon harvest in space and return to earth, however, all inflorescences of the flight-grown plants were sterile. To ascertain if Super Dwarf wheat responded to the 1.1 to 1.7 μmol · mol −1 atmospheric levels of ethylene measured on the Mir prior to and during flowering, plants on earth were exposed to 0, 1, 3, 10, and 20 μmol · mol −1 of ethylene gas and 1200 μmol · mol −1 CO 2 from 7 d after emergence to maturity. As in our Mir wheat, plant height, awn length, and the flag leaf were significantly shorter in the ethylene-exposed plants than in controls; inflorescences also exhibited 100 percnt; sterility. Scanning-electron-microscopic (SEM) examination of florets from Mir-grown and ethylene-treated, earth-grown plants showed that development ceased prior to anthesis, and the anthers did not dehisce. Laser scanning confocal microscopic (LSCM) examination of pollen grains from Mir and ethylene-treated plants on earth exhibited zero, one, and occasionally two, but rarely three nuclei; pollen produced in the absence of ethylene was always trinucleate, the normal condition. The scarcity of trinucleate pollen, abrupt cessation of floret development prior to anthesis, and excess tillering in wheat plants on Mir and in ethylene-containing atmospheres on earth build a strong case for the ethylene on Mir as the agent for the induced male sterility and other symptoms, rather than microgravity.