Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides

• Published in: Bioresource technology Vol. 89; no. 2; pp. 133 - 138
• Main Authors: Benimeli, C.S, Amoroso, M.J, Chaile, A.P, Castro, G.R
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.09.2003; Elsevier Science
• Subjects: Actinomycetes; Aldrin; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Chlordane; Environment and pollution; Freshwater; Fundamental and applied biological sciences. Psychology; Hydrocarbons, Chlorinated; Industrial applications and implications. Economical aspects; Insecticides - metabolism; Lindane; Organochlorine pesticides; Streptomycetaceae - isolation & purification; Streptomycetaceae - physiology; Streptomycetes; Water Purification - methods; Aldrin; Streptomycetes; Chlordane; Lindane; Organochlorine pesticides; Biodegradation; Microorganism growth; Insecticide; Streptomycetaceae; Pesticides; Actinomycetales; Synthetic medium; Organochlorine compounds; Microorganism culture; Bacteria; Actinomycetes; Xenobiotic
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(03)00061-0

Ninety-three wild-type isolates identified as actinomycetes were tested against 11 organochlorine pesticides (OPs): aldrin, chlordane, DDD, DDE, DDT, dieldrin, heptachlor, and heptachlor epoxides, lindane, and methoxychlor. Qualitative screening agar assays displayed 62–78% tolerance of strains to OPs. Four strains designed M4, M7, M9 and M15 were selected based on multi-OP-tolerance, and identified as members of the streptomycetes group. Different growth profiles were observed in cultures of the four selected streptomycetes cultured in synthetic medium containing 5–50 μg l −1 aldrin or chlordane or lindane. Increase of aldrin removal by the selected microorganisms was concomitant with the 4.8–36.0 μg l −1 pesticide concentration range. After 72 h of streptomycete M7 growth in synthetic medium containing 48.0 μg l −1 aldrin, the remaining OP concentration in the supernatant was approximately 10% of the initial concentration. Also, in stationary growth phase less than 2.5 μg l −1 aldrin residual concentration was detected in the medium.