KRAS Mutational Profiles among Colorectal Cancer Patients in the East Coast of Peninsular Malaysia

• Published in: Diagnostics (Basel) Vol. 13; no. 5; p. 822
• Main Authors: Hasbullah, Hidayati Husainy, Sulong, Sarina, Che Jalil, Nur Asyilla, Abdul Aziz, Ahmad Aizat, Musa, Nurfadhlina, Musa, Marahaini
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.02.2023; MDPI
• Subjects: Cancer therapies; Cell growth; codons 12 and 13; Colorectal cancer; Ethnicity; Genes; Hospitals; Kinases; KRAS; Monoclonal antibodies; Mutation; Pathology; Patients; Population; precision medicine; Proteins; Statistical analysis; Tumors; Kuala Lumpur Malaysia; Malaysia; Selangor Malaysia; United States > US; precision medicine; KRAS; colorectal cancer; codons 12 and 13
• ISSN: 2075-4418; 2075-4418
• DOI: 10.3390/diagnostics13050822

is a key driver gene in colorectal carcinogenesis. Despite this, there are still limited data on the mutational status of amongst colorectal cancer (CRC) patients in Malaysia. In the present study, we aimed to analyze the mutational profiles on codons 12 and 13 amongst CRC patients in Hospital Universiti Sains Malaysia, Kelantan, located on the East Coast of Peninsular Malaysia. DNA were extracted from formalin-fixed, paraffin-embedded tissues obtained from 33 CRC patients diagnosed between 2018 and 2019. Amplifications of codons 12 and 13 of were conducted using conventional polymerase chain reaction (PCR) followed by Sanger sequencing. Mutations were identified in 36.4% (12/33) of patients, with G12D (50%) being the most frequent single-point mutation observed, followed by G12V (25%), G13D (16.7%), and G12S (8.3%). No correlation was found between mutant and location of the tumor, staging, and initial carcinoembryonic antigen (CEA) level. Current analyses revealed that a significant proportion of CRC patients in the East Coast of Peninsular Malaysia have mutations, where this frequency is higher compared to those in the West Coast. The findings of this study would serve as a precursor for further research that explores mutational status and the profiling of other candidate genes among Malaysian CRC patients.