Participation of satellite glial cells of the dorsal root ganglia in acute nociception

• Published in: Neuroscience letters Vol. 676; pp. 8 - 12
• Main Authors: Lemes, Júlia Borges Paes, de Campos Lima, Tais, Santos, Débora Oliveira, Neves, Amanda Ferreira, de Oliveira, Fernando Silva, Parada, Carlos Almicar, da Cruz Lotufo, Celina Monteiro
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 29.05.2018
• Subjects: Acetamides - pharmacology; Adenosine triphosphate; Adenosine Triphosphate - metabolism; Animals; Calcium - metabolism; Capsaicin - pharmacology; Carbenoxolone - pharmacology; Ganglia, Spinal - physiology; Gap junctions; Gap Junctions - physiology; Male; Nociception; Nociception - physiology; Pain Measurement; Primary Cell Culture; Purinergic P2X Receptor Agonists - pharmacology; Purinergic P2X Receptor Antagonists - pharmacology; Purinergic P2X7 receptor; Quinolines - pharmacology; Rats, Wistar; Satellite Cells, Perineuronal - physiology; Satellite glial cells; Nociception; Purinergic P2X7 receptor; Gap junctions; Satellite glial cells; Adenosine triphosphate
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2018.04.003

•Nociceptors communicate with satellite glial cells in dorsal root ganglia through ATP release.•Activation of glial P2X7 receptors by ATP participates in acute nociception.•Communication through gap junctions in satellite glial cells is involved in acute pain. At dorsal root ganglia, neurons and satellite glial cells (SGC) can communicate through ATP release and P2X7 receptor activation. SGCs are also interconnected by gap junctions and have been previously implicated in modulating inflammatory and chronic pain.We now present evidence that SGCs are also involved in processing acute nociception in rat dorsal root ganglia. Using primary dorsal root ganglia cultures we observed that calcium transients induced in neurons by capsaicin administration were followed by satellite glial cells activation. Only satellite glial cells response was reduced by administration of the P2X7 receptor antagonist A740003. In vivo, acute nociception induced by intraplantar injection of capsaicin in rats was inhibited by A740003 or by the gap junction blocker carbenoxolone administered at the dorsal root ganglia (L5 level). Both drugs also reduced the second phase of the formalin test. These results suggest that communication between neurons and satellite glial cells is not only involved in inflammatory or pathological pain, but also in the transmission of the nociceptive signal, possibly in situations involving C-fiber activation.