COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)

• Published in: Bulletin of entomological research Vol. 105; no. 2; pp. 202 - 209
• Main Authors: Varadínová, Z., Wang, Y. J., Kučerová, Z., Stejskal, V., Opit, G., Cao, Y., Li, F. J., Li, Z. H.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.04.2015
• Subjects: Animals; Bar codes; Base Sequence; Coleoptera; Coleoptera - classification; Coleoptera - genetics; Cryptolestes; Cryptolestes ferrugineus; Cryptolestes pusillus; DNA Barcoding, Taxonomic; DNA Primers; Electron Transport Complex IV - genetics; Feasibility Studies; Food Parasitology; Free trade; Grain; Insects; International trade; Laemophloeidae; Larva - genetics; Molecular Sequence Data; Pest control; Pests; Polymerase Chain Reaction; Research Papers; Sequence Analysis, DNA; Asia; North America; Europe; flat grain beetles; COI barcode gene; species-specific primer; molecular diagnosis
• ISSN: 0007-4853; 1475-2670; 1475-2670
• DOI: 10.1017/S0007485315000024

Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).