Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus

• Published in: Journal of general virology Vol. 75; no. 2; pp. 259 - 267
• Main Authors: Solovyev, A. G, Novikov, V. K, Merits, A, Savenkov, E. I, Zelenina, D. A, Tyulkina, L. G, Morozov, S. Yu
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.02.1994; Society for General Microbiology
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Genes, Viral; Molecular Sequence Data; Open Reading Frames; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Potexvirus - classification; Potexvirus - genetics; Protein Biosynthesis; RNA, Viral - chemistry; Systematics. Structure, properties and multiplication. Genetics; Viral Proteins - chemistry; Genome organization; In vitro translation; Plant pathogen; Nucleotide sequence; Taxonomy; Potexvirus; Chemotaxonomy; Homology; Genetical translation; Phylogeny; Dendrogram; Virus; Single stranded RNA; Aminoacid sequence; Structural analysis
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-2-259

1 Department of Virology, Moscow State University, Moscow 119899 and 2 Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia The complete nucleotide sequence of Plantago asiatica mosaic virus (P1AMV) genomic RNA has been determined. The 6128 nucleotide sequence contains five open reading frames (ORFs) coding for proteins of M r 156K (ORF1), 25K (ORF2), 12K (ORF3), 13K (ORF4) and 22K (ORF5). The sequences of these P1AMV proteins exhibit strong homology to the proteins of the other potexviruses. Phylogenetic trees based on the multiple sequence alignments of three conserved domains in ORF1 product and capsid protein reveal a close relationship of P1AMV to papaya mosaic virus and clover yellow mosaic virus. The P1AMV genomic RNA and a major subgenomic RNA (sgRNA) of 0.9 kb have been detected in infected leaves by Northern blot hybridization. The latter sgRNA is the messenger for virus capsid protein and its 5' terminus has been located 23 nucleotides upstream of the initiator codon of the coat protein gene. The P1AMV virion RNA and RNA transcript resembling the 0.9 kb sgRNA have been translated in vitro giving rise to a single major 170K product and a major 22K product, respectively. Received 17 June 1993; accepted 21 September 1993.