Expression Signatures of Long Noncoding RNAs in Adolescent Idiopathic Scoliosis

• Published in: BioMed research international Vol. 2015; no. 2015; pp. 1 - 9
• Main Authors: Zhuang, Qian-yu, Yu, Bin, Wang, Liang, Liu, Xiao-Yang, Wang, Yi-Peng
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2015; Hindawi Limited
• Subjects: Adolescent; Bone surgery; Disease; Female; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Gene Regulatory Networks; Genomes; Hospitals; Humans; Hybridization; Musculoskeletal system; Pathogenesis; Proteins; Real-Time Polymerase Chain Reaction; Reproducibility of Results; RNA polymerase; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Scoliosis; Scoliosis - genetics; Software; Studies
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2015/276049

Purpose. Adolescent idiopathic scoliosis (AIS), the most common pediatric spinal deformity, is considered a complex genetic disease. Causing genes and pathogenesis of AIS are still unclear. This study was designed to identify differentially expressed long noncoding RNAs (lncRNAs) involving the pathogenesis of AIS. Methods. We first performed comprehensive screening of lncRNA and mRNA in AIS patients and healthy children using Agilent human lncRNA + mRNA Array V3.0 microarray. LncRNAs expression in different AIS patients was further evaluated using quantitative PCR. Results. A total of 139 lncRNAs and 546 mRNAs were differentially expressed between AIS patients and healthy control. GO and Pathway analysis showed that these mRNAs might be involved in bone mineralization, neuromuscular junction, skeletal system morphogenesis, nucleotide and nucleic acid metabolism, and regulation of signal pathway. Four lncRNAs (ENST00000440778.1, ENST00000602322.1, ENST00000414894.1, and TCONS_00028768) were differentially expressed between different patients when grouped according to age, height, classification, severity of scoliosis, and Risser grade. Conclusions. This study demonstrates the abnormal expression of lncRNAs and mRNAs in AIS, and the expression of some lncRNAs was related to clinical features. This study is helpful for further understanding of lncRNAs in pathogenesis, treatment, and prognosis of AIS.