The Type of Basal Promoter Determines the Regulated or Constitutive Mode of Transcription in the Common Control Region of the Yeast Gene Pair GCY1/RIO1

• Published in: The Journal of biological chemistry Vol. 272; no. 50; pp. 31630 - 31635
• Main Authors: Angermayr, Michaela, Bandlow, Wolfhard
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.12.1997; American Society for Biochemistry and Molecular Biology
• Subjects: ADN; ADN RECOMBINADO; ADN RECOMBINE; ALDO-KETO REDUCTASE; BETA GALACTOSIDASA; BETA GALACTOSIDASE; BINDING; Binding Sites; Chromosome Inversion; Chromosome Mapping; Consensus Sequence; CRYSTALLIN LIKE YEAST PROTEIN; DNA; DNA-Binding Proteins; EXPRESION GENICA; EXPRESSION DES GENES; FACTEUR DE TRANSCRIPTION; FACTORES DE TRANSCRIPCION; GALACTOSA; GALACTOSE; Galactose - pharmacology; GCY1 GENE; GENE; GENE EXPRESSION; GENES; Genes, Fungal; GENETIC REGULATION; GENETICA; GENETICS; GENETIQUE; GTP-Binding Proteins; GTP-Binding Proteins - metabolism; INTERGENIC DNA; metabolism; NUCLEOTIDE SEQUENCE; Oligodeoxyribonucleotides; Oligodeoxyribonucleotides - metabolism; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; pharmacology; Poly dA-dT; Poly dA-dT - metabolism; Promoter Regions, Genetic; PROTEINAS; PROTEINE; PROTEINS; rap GTP-Binding Proteins; RECOMBINANT DNA; REPORTER GENES; RIO1 GENE; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; TATA BOX; TRANSCRIPCION; TRANSCRIPTION; transcription (genetics); TRANSCRIPTION FACTORS; Transcription Factors - metabolism; Transcription, Genetic
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.272.50.31630

The yeast genes, GCY1 andRIO1, are transcribed divergently from the 869-base pair intergenic region. GCY1 is inducible by galactose about 25-fold due to Gal4p-binding to a singleUASGAL, whereas RIO1 is constitutively expressed. GCY1 has a TATA box obeying the consensus TATAAA, whereas the RIO1 5′-upstream region lacks such a motif. In vitro mutagenesis of the TATA motif ofGCY1, on the one hand, and introduction of a TATA-element into the promoter of RIO1, on the other hand, as well as inversion of the intergenic region have revealed that transcription ofGCY1 and RIO1 is only regulated by Gal4p when a consensus TATA motif is included in their core promoters but not in its absence. The data imply that only transcription complexes that assemble at a consensus TATA box are compatible with specific transactivators, such as Gal4p. As a result, the adjacent gene is subject to regulated expression. By contrast, if a consensus TATA sequence is absent, the initiation complex does not respond to regulatory transcription factors, and consequently, the respective gene is constitutively transcribed. On the other hand, we show that two blocks of homo-oligomeric (dA·dT) sequences do not function as boundary sequences that might confine regulatory action of Gal4p toGCY1.