TSLP Production by Epithelial Cells Exposed to Immunodeficiency Virus Triggers DC-Mediated Mucosal Infection of CD4⁺ T Cells
Mucosal dendritic cells have been implicated in the capture, storage, and transmission of HIV to CD4⁺T cells as well as in the promotion of HIV replication in activated CD4⁺T cells during the cognate T-cell and DC interaction. We report that HIV induces human genital mucosal epithelial cells to prod...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 39; pp. 16776 - 16781 |
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Main Authors | , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
29.09.2009
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Mucosal dendritic cells have been implicated in the capture, storage, and transmission of HIV to CD4⁺T cells as well as in the promotion of HIV replication in activated CD4⁺T cells during the cognate T-cell and DC interaction. We report that HIV induces human genital mucosal epithelial cells to produce thymic stromal lymphopoietin (TSLP) via activation of the NFKB signaling pathway. The TSLP secreted by HIV exposed epithelial cells activated DC, which promoted proliferation and HIV-1 replication of co-cultured autologous CD4⁺T cells. In rhesus macaques, we observed dramatic increases in TSLP expression concurrent with an increase in viral replication in the vaginal tissues within the first 2 weeks after vaginal SIV exposure. These data suggest that HIV-mediated TSLP production by mucosal epithelial cells is a critical trigger for DC-mediated amplification of HIV-infection in activated CD4⁺T cells. The cross talk between mucosal epithelial cells and DC mediated by HIV-induced TSLP, may be an important mechanism for the high rate of HIV infection in women through the vaginal mucosa. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: D.F., S.F.Z., C.J.M., S.-C.S., Y.-J.L., and K.J.S. designed research; D.F., H.H., S.H., P.N.N., M.Z., M.C., B.N., Yui-Hsi Wang, Yi-Hong Wang, Z.-m.M., H.-C.L., and A.N.C. performed research; and D.F. wrote the paper. 1D.F., H.H., and S.H. contributed equally to this work. Edited by Ralph M. Steinman, The Rockefeller University, New York, NY, and approved August 18, 2009 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0907347106 |