Phase I/II study of pegylated arginine deiminase (ADI-PEG 20) in patients with advanced melanoma

• Published in: Investigational new drugs Vol. 31; no. 2; pp. 425 - 434
• Main Authors: Ott, Patrick A., Carvajal, Richard D., Pandit-Taskar, Neeta, Jungbluth, Achim A., Hoffman, Eric W., Wu, Bor-Wen, Bomalaski, John S., Venhaus, Ralph, Pan, Linda, Old, Lloyd J., Pavlick, Anna C., Wolchok, Jedd D.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.04.2013; Springer; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Antineoplastic agents; Biological and medical sciences; Cancer therapies; Clinical trials; Cohort Studies; Dermatology; Dose-Response Relationship, Drug; Drug dosages; Enzymes; Female; Follow-Up Studies; General aspects; Humans; Hydrolases - pharmacokinetics; Hydrolases - therapeutic use; Immunoenzyme Techniques; Immunotherapy; Male; Maximum Tolerated Dose; Medical research; Medical sciences; Medicine; Medicine & Public Health; Melanoma; Melanoma - drug therapy; Melanoma - metabolism; Melanoma - pathology; Metabolism; Metastasis; Middle Aged; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplasm Staging; Oncology; Patients; Pharmacology; Pharmacology. Drug treatments; Pharmacology/Toxicology; Phase II Studies; Polyethylene glycol; Polyethylene Glycols - pharmacokinetics; Polyethylene Glycols - therapeutic use; Prognosis; Skin Neoplasms - drug therapy; Skin Neoplasms - metabolism; Skin Neoplasms - secondary; Studies; Survival Rate; Tissue Distribution; Toxicity; Tumors; Tumors of the skin and soft tissue. Premalignant lesions; Uveal Neoplasms - drug therapy; Uveal Neoplasms - metabolism; Uveal Neoplasms - secondary; United States > US; Auxotrophy; Argininosuccinate synthetase; Metastatic; Melanoma; Arginine deiminase; Human; Carbon-nitrogen ligases; Enzyme; Toxicity; Argininosuccinate synthase; Malignant tumor; Metastasis; Ethylene oxide polymer; Cyclic ether polymer; Ligases; Phase II trial; Malignant melanoma; Phase I trial; Hydrolases; Advanced stage; Pegylated form; Cancer
• ISSN: 0167-6997; 1573-0646
• DOI: 10.1007/s10637-012-9862-2

Summary Background Arginine deiminase (ADI) is an enzyme that degrades arginine, an amino acid that is important for growth and development of normal and neoplastic cells. Melanoma cells are auxotrophic for arginine, because they lack argininosuccinatesynthetase (ASS), a key enzyme required for the synthesis of arginine. Patients and methods Patients with advanced melanoma were treated with 40, 80 or 160 IU/m 2 ADI-PEG 20 i.m. weekly. Primary endpoints were toxicity and tumor response, secondary endpoints included metabolic response by 18 FDG-PET, pharmacodynamic (PD) effects upon circulating arginine levels, and argininosuccinate synthetase tumor expression by immunohistochemistry. Results 31 previously treated patients were enrolled. The main toxicities were grade 1 and 2 adverse events including injection site pain, rash, and fatigue. No objective responses were seen. Nine patients achieved stable disease (SD), with 2 of these durable for >6 months. Four of the 9 patients with SD had uveal melanoma. PD analysis showed complete plasma arginine depletion in 30/31 patients by day 8. Mean plasma levels of ADI-PEG 20 correlated inversely with ADI-PEG 20 antibody levels. Immunohistochemical ASS expression analysis in tumor tissue was negative in 24 patients, whereas 5 patients had <5 % cells positive. Conclusions ADI-PEG 20 is well tolerated in advanced melanoma patients and leads to consistent, but transient, arginine depletion. Although no RECIST responses were observed, the encouraging rate of SD in uveal melanoma patients indicates that it may be worthwhile to evaluate ADI-PEG 20 in this melanoma subgroup.