Pilot Study on the Action of Thymus vulgaris Essential Oil in Treating the Most Common Bacterial Contaminants and Salmonella enterica subsp. enterica Serovar Derby in Poultry Litter

• Published in: Antibiotics (Basel) Vol. 12; no. 3; p. 436
• Main Authors: Galgano, Michela, Pellegrini, Francesco, Fracchiolla, Giuseppe, Mrenoshki, Daniela, Zarea, Aya Attia Koraney, Bianco, Angelica, Del Sambro, Laura, Capozzi, Loredana, Schiavone, Antonella, Saleh, Medhat S, Camero, Michele, Tempesta, Maria, Cirone, Francesco, Buonavoglia, Domenico, Pratelli, Annamaria, Buonavoglia, Alessio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.02.2023; MDPI
• Subjects: Agricultural wastes; Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Aqueous solutions; Bacteria; Bedding; Contaminants; Disinfection & disinfectants; Drug resistance; E coli; Environmental impact; Escherichia coli; Essential oils; Farms; Genes; Genomes; Gram-positive bacteria; Lactose; Litter; Mammaliicoccus lentus; Microbial contamination; Microorganisms; Oils & fats; Pathogens; Poultry; Poultry farming; poultry farms; Reduction; Salmonella; Salmonella Derby; poultry farms; Mammaliicoccus lentus; Salmonella Derby; antimicrobial activity; essential oils; Escherichia coli
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics12030436

The indiscriminate use of antimicrobials in poultry farms is linked to the increase in multi-resistant bacteria. Accordingly, based on the antimicrobial properties of Thyme Essential Oil (TEO), the present study evaluated the effects of TEO on the reduction of common microbial contaminants and on poultry litter. A litter bulk sample was collected in a broiler farm and qualitative/quantitative investigations identified and The experimental contamination with Derby wild strain was also performed. All pathogens showed phenotypic and genotypic resistance to different classes of antibiotics. The litter, split in different units, was treated with aqueous solutions of TEO at different concentrations (5% to 1.25%), demonstrating its effectiveness in reducing the total number of bacteria. The strongest antibacterial action was observed at the lowest concentration against , with a growth reduction compared to the positive control of 73.3% and 77.8% against and Derby, respectively, while towards . the reduction was 50%. Our data confirm the antimicrobial activity of TEO and suggest its possible application for the treatment of poultry litter as an effective and natural approach for the prevention of diseases caused by the most common bacteria that colonize poultry farms, counteracting the onset of antibiotic resistance.