Impact of Water Activity and Temperature on Growth and Alternariol and Alternariol Monomethyl Ether Production of Alternaria alternata Isolated from Soybean

• Published in: Journal of food protection Vol. 73; no. 2; pp. 336 - 343
• Main Authors: OVIEDO, M. S, RAMIREZ, M. L, BARROS, G. G, CHULZE, S. N
• Format: Journal Article
• Language: English
• Published: Des Moines, IA International Association for Food Protection 01.02.2010; Elsevier Limited
• Subjects: Alternaria - growth & development; Alternaria - metabolism; Alternaria alternata; Biological and medical sciences; Colony Count, Microbial; Consumer Product Safety; Food Contamination - analysis; Food industries; Food microbiology; Food safety; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Glycerol; Glycine max - microbiology; Lactones - metabolism; Metabolites; Mycotoxins; Mycotoxins - biosynthesis; Pathogens; Seeds; Solvents; Soybeans; Temperature; Time Factors; Toxicology; Toxins; Water; Water - metabolism; Water availability; Argentina; Fungi; Grain legume; Temperature; Water activity; Ether; Growth; Production; Fungi Imperfecti; Alternaria alternata; Soybean
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028x-73.2.336

The objective of this study was to determine the effect of water activity (a(w); 0.995, 0.98, 0.96, 0.94, 0.92, and 0.90), temperature (5, 18, 25, and 30 degrees C), incubation time (7 to 35 days), and their interactions on mycelial growth and alternariol (AOH) and alternariol monomethyl ether (AME) production. Two Alternaria alternata strains isolated from soybeans in Argentina were grown on 2% soybean extract agar. Maximum growth rates were obtained at the highest a(w) (0.995) and 25 degrees C, with growth decreasing as the water availability of the medium was reduced. Maximum amount of AOH was produced at 0.98 a(w) and 25 degrees C for both strains. Maximum AME production was obtained for both strains at 30 degrees C but different a(w) values, 0.92 and 0.94, for the strains RC 21 and RC 39, respectively. The concentrations of both toxins varied considerably depending on the a(w) and temperature interactions assayed. The two metabolites were produced from 5 to 30 degrees C and at a(w) values of 0.92 to 0.995. Although at 5 and 18 degrees C little mycotoxin was produced at a(w) lower than 0.94. Two-dimensional profiles of a(w) by temperature interactions were developed from these data to identify areas where conditions indicate a significant risk from AOH and AME accumulation on soybeans. All the conditions of a(w) and temperature that resulted in maximum production of both toxins are those found during soybean development in the field. Thus, field conditions are likely to be conducive to optimum A. alternata growth and toxin production.