Maternal Primary Imprinting Is Established at a Specific Time for Each Gene throughout Oocyte Growth
Primary imprinting during gametogenesis governs the monoallelic expression/repression of imprinted genes in embryogenesis. Previously, we showed that maternal primary imprinting is disrupted in neonate-derived non-growing oocytes. Here, to investigate precisely when and in what order maternal primar...
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Published in | The Journal of biological chemistry Vol. 277; no. 7; pp. 5285 - 5289 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
15.02.2002
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Subjects | |
Online Access | Get full text |
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Summary: | Primary imprinting during gametogenesis governs the monoallelic expression/repression of imprinted genes in embryogenesis.
Previously, we showed that maternal primary imprinting is disrupted in neonate-derived non-growing oocytes. Here, to investigate
precisely when and in what order maternal primary imprinting progresses, we produced parthenogenetic embryos containing one
genome from a non-growing or growth-stage oocyte from 1- to 20-day-old mice and one from a fully grown oocyte of adult mice.
We used these embryos to analyze the expression of eight imprinted genes: Peg1/Mest , Peg3 , Snrpn , Znf127 , Ndn , Impact , Igf2r , and p57 KIP2 . The results showed that the imprinting signals for each gene were not all imposed together at a specific time during oocyte
growth but rather occurred throughout the period from primary to antral follicle stage oocytes. The developmental ability
of the constructed parthenogenetic embryos was gradually reduced as the nuclear donor oocytes grew. These studies provide
the first insight into the process of primary imprinting during oocyte growth. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M108586200 |