Diagnostic value of elevated serum miRNA-143 levels in sepsis

• Published in: Journal of international medical research Vol. 44; no. 4; pp. 875 - 881
• Main Authors: Han, Yu, Dai, Qing-Chun, Shen, Hong-Li, Zhang, Xiao-Wei
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.08.2016
• Subjects: Adult; Aged; Demography; Female; Humans; Male; MicroRNAs - blood; Middle Aged; Research Reports; ROC Curve; Sepsis - blood; Sepsis - diagnosis; Sepsis - genetics; Systemic Inflammatory Response Syndrome - blood; Systemic Inflammatory Response Syndrome - diagnosis; Systemic Inflammatory Response Syndrome - genetics; Young Adult; MicroRNA-143; diagnosis; sepsis
• ISSN: 0300-0605; 1473-2300; 1473-2300
• DOI: 10.1177/0300060516645003

Objective To evaluate serum micro RNA-143 (miR-143) levels in patients with sepsis or non-infectious systemic inflammatory response syndrome (SIRS), and investigate its possible diagnostic or prognostic value. Methods Serum was obtained from patients with sepsis or SIRS and healthy control subjects. Relative miR-143 expression was determined using quantitative real time polymerase chain reaction. The diagnostic and prognostic value of serum miR-143 was evaluated. Results Serum miR-143 levels were significantly higher in patients with sepsis (n = 103) than patients with SIRS (n = 95) and healthy controls (n = 40). There were significant positive correlations between serum miR-143 level and SOFA and APACHE II scores in patients with sepsis (r = 0.794 and r = 0.825, respectively). Serum miR-143 had a sensitivity of 78.6% and specificity of 91.6% for distinguishing between sepsis and SIRS. There was no association between serum miR-143 and 28-day survival in patients with sepsis. Conclusion Serum miR-143 is elevated in patients with sepsis, and may be a useful biomarker for distinguishing between sepsis and SIRS.