Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst

• Published in: BMC developmental biology Vol. 19; no. 1; p. 15
• Main Authors: Zolini, Adriana M, Negrón-Pérez, Verónica M, Hansen, Peter J
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 05.07.2019; BioMed Central
• Subjects: Androgen-Binding Protein - genetics; Androgens; Animals; Apoptosis; Beef cattle; Biochemistry; Blastocyst - cytology; Cattle; Cell Differentiation - genetics; Embryo; Embryonic development; Embryonic Development - genetics; Endoplasmic Reticulum Stress - genetics; Gene expression; Gene Expression Regulation, Developmental - genetics; Genes; Genetic polymorphisms; Messenger RNA; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism; Polymorphism, Single Nucleotide - genetics; ras GTPase-Activating Proteins - metabolism; Receptors, CCR2 - metabolism; Receptors, CCR7 - metabolism; RNA; RNA, Messenger - genetics; Single nucleotide polymorphisms; STAT3 Transcription Factor - metabolism; Tight Junction Proteins - metabolism; Tunicamycin - pharmacology; Development; Embryo; PARM1; Gene expression; Blastocyst; ER stress response
• ISSN: 1471-213X; 1471-213X
• DOI: 10.1186/s12861-019-0195-7

Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the blastocyst stage. Here we tested the importance of PARM1 for development by evaluating consequences of reducing PARM1 mRNA abundance on embryonic development and differentiation, gene expression and resistance to ER stress. Knockdown of PARM1 using an anti-PARM1 GapmeR did not affect competence of embryos to develop into blastocysts but decreased the number of trophectoderm (TE) cells in the blastocyst and tended to increase the number of cells in the blastocyst inner cell mass (ICM). Treatment of embryos with anti-PARM1 GapmeR affected expression of 4 and 3 of 90 genes evaluated at the compact-morula and blastocyst stage of development at days 5.5 and 7.5 after fertilization, respectively. In morulae, treatment increased expression of DAB2, INADL, and STAT3 and decreased expression of CCR2. At the blastocyst stage, knockdown of PARM1 increased expression of PECAM and TEAD4 and decreased expression of CCR7. The potential role of PARM1 in ER stress response was determined by evaluating effects of knockdown of PARM1 on development of embryos after exposure to heat shock or tunicamycin and on expression of ATF6, DDIT3 and EIF2AK3 at the compact morula and blastocyst stages. Both heat shock and tunicamycin reduced the percent of embryos becoming a blastocyst but response was unaffected by PARM1 knockdown. Similarly, there was no effect of knockdown on steady-state amounts of ATF6, DDIT3 or EIF2AK3. PARM1 participates in formation of TE and ICM cells in early embryonic development but there is no evidence for the role of PARM1 in the ER stress response.