Evaluation of adhesion properties and antibacterial activities of the infant gut commensal Bifidobacterium bifidum PRL2010

• Published in: Anaerobe Vol. 21; pp. 9 - 17
• Main Authors: Serafini, Fausta, Strati, Francesco, Ruas-Madiedo, Patricia, Turroni, Francesca, Foroni, Elena, Duranti, Sabrina, Milano, Francesco, Perotti, Alessio, Viappiani, Alice, Guglielmetti, Simone, Buschini, Annamaria, Margolles, Abelardo, van Sinderen, Douwe, Ventura, Marco
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2013
• Subjects: adhesion; Anti-Bacterial Agents - pharmacology; antibacterial properties; Antibiosis; bacteria; Bacterial Adhesion; Bifidobacteria; Bifidobacterium - drug effects; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Bifidobacterium - physiology; Bifidobacterium bifidum; Bile Acids and Salts - pharmacology; Caco-2 Cells; Cronobacter sakazakii; Cronobacter sakazakii - physiology; Epithelial Cells - microbiology; Escherichia coli; Escherichia coli - physiology; feces; Feces - microbiology; food industry; Gastrointestinal Tract - microbiology; health promotion; Host-Pathogen Interactions; HT29 Cells; Humans; Hydrogen-Ion Concentration; Infant; Intestinal adhesion; intestines; Intestines - microbiology; Microbial Sensitivity Tests; Pathogen inhibition; Probiotic; Probiotics; Sodium Chloride - pharmacology; Probiotic; Intestinal adhesion; Bifidobacteria; Pathogen inhibition
• ISSN: 1075-9964; 1095-8274; 1095-8274
• DOI: 10.1016/j.anaerobe.2013.03.003

Bifidobacteria are extensively exploited by the food industry as health-promoting microorganisms. However, very little is known about the molecular mechanisms responsible for these beneficial activities, or the molecular players that sustain their ability to colonize and persist within the human gut. Here, we have investigated the enteric adaptation features of the gut commensal Bifidobacterium bifidum PRL2010, originally isolated from infant feces. This strain was able to survive under gastrointestinal challenges, while it was shown to adhere to human epithelial intestinal cell monolayers (Caco 2 and HT-29), thereby inhibiting adhesion of pathogenic bacteria such as Escherichia coli and Cronobacter sakazakii. •Bifidobacteria are exploited by the food industry as probiotic microorganisms.•There is a large paucity of data about their capabilities to colonize the human host.•Here we have characterized an infant gut commensal bifidobacterial strain.•This strain was shown to be able to survive under gastrointestinal challenges.•It is able to adhere to human epithelial intestinal cell.