Malt sprout extract medium for cultivation of Lactobacillus plantarum protective cultures

• Published in: Letters in applied microbiology Vol. 39; no. 4; pp. 336 - 340
• Main Authors: Laitila, A, Saarela, M, Kirk, L, Siika-aho, M, Haikara, A, Mattila-Sandholm, T, Virkajarvi, I
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2004; Blackwell Science
• Subjects: Anti-Bacterial Agents - pharmacology; Antifungal Agents - pharmacology; antimicrobial; biocontrol; Biological and medical sciences; Biotechnology - methods; Colony Count, Microbial; cultivation; Culture Media - chemistry; Culture Media, Conditioned - pharmacology; Edible Grain - chemistry; Fundamental and applied biological sciences. Psychology; Fusarium - drug effects; Fusarium avenaceum; growth medium; Hydrogen-Ion Concentration; Lactobacillus - growth & development; Lactobacillus - metabolism; Lactobacillus plantarum; malt sprouts; Microbial Sensitivity Tests; Microbiology; Pantoea - drug effects; Pantoea agglomerans; Plant Extracts - chemistry; Culture medium; antimicrobial; Lactic acid bacteria; malt sprouts; Lactobacillus plantarum; Growth; Applied microbiology; Biological control; Extract; growth medium; Antimicrobial agent; cultivation; Malt; Bacteria; Lactobacillaceae; Protection; biocontrol; Culture
• ISSN: 0266-8254; 1472-765X; 1365-2673
• DOI: 10.1111/j.1472-765X.2004.01579.x

Aims:  The aim was to develop a cheap cereal‐based alternative medium for the large‐scale production of biopreservative Lactobacillus plantarum VTT E‐79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E‐79098 and the antimicrobial activity of the cell‐free extracts. Methods:  Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell‐free extracts against Pantoea agglomerans VTT E‐90396 and Fusarium avenaceum VTT D‐80147 was assessed with automated turbidometry. Significance and Impact of the Study:  When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E‐79098 and enhanced antimicrobial potential against the Gram‐negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low‐cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.