Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil

• Published in: FEMS microbiology letters Vol. 265; no. 1; pp. 118 - 125
• Main Authors: Yang, Chao, Liu, Na, Guo, Xinmin, Qiao, Chuanling
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2006; Blackwell; Oxford University Press
• Subjects: Bacteria; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Bioremediation; Biotechnology; Carbon; Chlorpyrifos; Chlorpyrifos - metabolism; Cloning; Cloning, Molecular; Escherichia coli - enzymology; Escherichia coli - genetics; Fenitrothion; Fundamental and applied biological sciences. Psychology; Gene sequencing; Genes, Bacterial; Genetics; Hydrolase; Inoculation; Insecticides; Insecticides - metabolism; Methyl parathion; Microbiology; Mission oriented research; mpd gene; Organophosphorus pesticides; Parathion; Pesticides; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Phosphorus; Phylogeny; rRNA 16S; Sludge; Soil bacteria; Soil contamination; Soil Microbiology; Soil microorganisms; Soil Pollutants - metabolism; Soil pollution; Soil treatment; Soils; Stenotrophomonas; Stenotrophomonas - genetics; Stenotrophomonas - growth & development; Stenotrophomonas - isolation & purification; Stenotrophomonas - metabolism; Trichloro-2-pyridinol; Wastewater treatment; organophosphorus pesticides; bioremediation; chlorpyrifos; gene; Pseudomonadales; Chlorpyrifos; Biodegradation; Insecticide; Stenotrophomonas; mpd gene; Pesticides; Contamination; Strain; Soils; Gene; Bacteria; Pseudomonadaceae; Organophosphorus compounds; Bioremediation
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2006.00478.x

Abstract An effective chlorpyrifos-degrading bacterium (named strain YC-1) was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences and BIOLOG test, strain YC-1 was identified as the genus Stenotrophomonas. The isolate utilized chlorpyrifos as the sole source of carbon and phosphorus for its growth and hydrolyzed chlorpyrifos to 3,5,6-trichloro-2-pyridinol. Parathion, methyl parathion, and fenitrothion also could be degraded by strain YC-1 when provided as the sole source of carbon and phosphorus. The gene encoding the organophosphorus hydrolase was cloned using a PCR cloning strategy based on the known methyl parathion degrading (mpd) gene of Plesiomonas sp. M6. Sequence blast result indicated this gene has 99% similar to mpd. The inoculation of strain YC-1 (106 cells g−1) to soil treated with 100 mg kg−1 chlorpyrifos resulted in a higher degradation rate than in noninoculated soils. Theses results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.