Intraluminal injection of short chain fatty acids diminishes intestinal mucosa injury in experimental ischemia-reperfusion

• Published in: Acta cirúrgica brasileira Vol. 21; no. 1; pp. 21 - 25
• Main Authors: Aguilar-Nascimento, José Eduardo de, Salomão, Alberto Bicudo, Nochi, Jr, Rubens Jardim, Nascimento, Mariana, Neves, José de Souza
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2006
• Subjects: Animals; Disease Models, Animal; Fatty Acids, Volatile - therapeutic use; Ileum - blood supply; Ileum - drug effects; Intestinal Mucosa - drug effects; Intestinal Mucosa - injuries; Intestine, Small; Ischemia; Jejunum - blood supply; Jejunum - drug effects; Leukocyte Count; Mucous, Membrane; Neutrophils; Neutrophils - drug effects; Random Allocation; Rats; Rats, Wistar; Reperfusion; Reperfusion Injury - blood; Reperfusion Injury - drug therapy; short-chain Fatty Acids, Volatile
• ISSN: 0102-8650; 0102-8650; 1678-2674
• DOI: 10.1590/s0102-86502006000100006

Investigated the effect of intraluminal short-chain fatty acids (SCFA) on the intestinal mucosa in the presence of ischemia-reperfusion injury (IRI). Six blind sacs of the small bowel (3 at the jejunum and 3 at the ileum) were created in ten Wistar rats. The lateral sacs of both bowel regions were subjected to IRI (15/15 minutes) while the medial sacs were let free to receive blood supply. In the lateral sacs, it was injected either a solution containing SCFA (butyrate, propionate and acetate) or pure saline at the bowel lumen. No fluid was injected in the medial sacs. Both at the jejunum and at the ileum the score of the mucosal injury was higher in saline than in control sacs. SCFA treated sacs showed lesser score at the ileum (p = 0.03) but were not significantly different at the jejunum (p = 0.83) when compared with saline sacs. It was found a significant greater number of neutrophils (p < 0.01) in the sacs treated with saline than in the other two sacs in both regions. SCFA protect the distal small bowel mucosa and diminishes infiltration of neutrophils to the gut lamina propria in IRI.