Perturbation of syntrophic isobutyrate and butyrate degradation with formate and hydrogen

• Published in: Biotechnology and bioengineering Vol. 52; no. 3; pp. 404 - 411
• Main Authors: Wu, Wei-Min, Jain, Mahendra K., Hickey, Robert F., Zeikus, J. Gregory
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05.11.1996; Wiley
• Subjects: anaerobic digestion; Biological and medical sciences; Biological treatment of waters; Biotechnology; butyrate; Environment and pollution; formate; Fundamental and applied biological sciences. Psychology; hydrogen; Industrial applications and implications. Economical aspects; isobutyrate; isomerization; Biodegradation; Archaeobacteria; Hydrogen; Isomerization; Mixed microorganism culture; Methanosarcinaceae; Anaerobic digestion; Butyrate; Methanomicrobiales; Methanogenic bacteria; Methanobacteriaceae; Bacteria; Formate; Inhibition; Methanobacteriales
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(19961105)52:3<404::AID-BIT6>3.0.CO;2-O

The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate–butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and hydrogen were used to perturb syntrophic butyrate and isobutyrate degradation by the culture. The reversible isomerization between isobutyrate and butyrate was inhibited by the addition of either formate or hydrogen, indicating that the isomerization was coupled with syntrophic butyrate degradation for the culture studied. Energetic analysis indicates that the direction of isomerization between isobutyrate and butyrate is controlled by the ratio between the two acids, and the most thermodynamically favorable condition for the degradation of butyrate or isobutyrate in conjunction with the isomerization is at almost equal concentrations of isobutyrate and butyrate. The degradation of isobutyrate and butyrate was completely inhibited in the presence of a high hydrogen partial pressure (>2000 Pa) or a measurable level of formate (10 μM or higher). Significant formate (more than 1 mM) was detected during the perturbation with hydrogen (17 to 40 kPa). Resumption of butyrate and isobutyrate degradation was related to the removal of formate. Energetic analysis supported that formate was another electron carrier, besides hydrogen, during syntrophic isobutyrate–butyrate degradation by this culture. © 1996 John Wiley & Sons, Inc.