Phase II clinical trial of peptide cocktail therapy for patients with advanced pancreatic cancer: VENUS‐PC study

• Published in: Cancer science Vol. 108; no. 1; pp. 73 - 80
• Main Authors: Suzuki, Nobuaki, Hazama, Shoichi, Iguchi, Haruo, Uesugi, Kazuhiro, Tanaka, Hiroaki, Hirakawa, Kosei, Aruga, Atsushi, Hatori, Takashi, Ishizaki, Hidenobu, Umeda, Yuzo, Fujiwara, Toshiyoshi, Ikemoto, Tetsuya, Shimada, Mitsuo, Yoshimatsu, Kazuhiko, Shimizu, Ryoichi, Hayashi, Hiroto, Sakata, Koichiro, Takenouchi, Hiroko, Matsui, Hiroto, Shindo, Yoshitaro, Iida, Michihisa, Koki, Yasunobu, Arima, Hideki, Furukawa, Hiroyuki, Ueno, Tomio, Yoshino, Shigefumi, Nakamura, Yusuke, Oka, Masaaki, Nagano, Hiroaki
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.01.2017; John Wiley and Sons Inc
• Subjects: Adult; Advanced pancreatic cancer; Aged; Aged, 80 and over; Antigens; Antineoplastic Combined Chemotherapy Protocols - administration & dosage; Antineoplastic Combined Chemotherapy Protocols - adverse effects; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Cancer therapies; Cancer vaccines; Cancer Vaccines - administration & dosage; Cancer Vaccines - adverse effects; Cancer Vaccines - immunology; Cancer Vaccines - therapeutic use; Chemotherapy; Clinical trials; CTL; Cytotoxicity; Deoxycytidine - administration & dosage; Deoxycytidine - adverse effects; Deoxycytidine - analogs & derivatives; Deoxycytidine - therapeutic use; Disease control; Disease-Free Survival; Female; Gemcitabine; Genotypes; Histocompatibility antigen HLA; HLA-A24 Antigen - genetics; HLA-A24 Antigen - immunology; Humans; Immune response; Immunization; Immunogenicity; Immunology; Immunotherapy; Injection; Kinesins - immunology; Lymphocytes; Lymphocytes T; Male; Medical prognosis; Middle Aged; Mortality; NMR; Nuclear magnetic resonance; Original; Pancreatic cancer; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - immunology; Patients; peptide cocktail; Peptides; Peptides - administration & dosage; Peptides - adverse effects; Peptides - immunology; Peptides - therapeutic use; phase II; Prognosis; R&D; Research & development; Response rates; T-Lymphocytes, Cytotoxic - immunology; Time Factors; Treatment Outcome; Vaccines; Vascular Endothelial Growth Factor Receptor-1 - immunology; Vascular Endothelial Growth Factor Receptor-2 - immunology; United States > US; Japan; phase II; CTL; Advanced pancreatic cancer; peptide cocktail; immunotherapy
• ISSN: 1347-9032; 1349-7006
• DOI: 10.1111/cas.13113

We previously conducted a phase I clinical trial combining the HLA‐A*2402‐restricted KIF20A‐derived peptide vaccine with gemcitabine for advanced pancreatic cancer (PC) and confirmed its safety and immunogenicity in cancer patients. In this study, we conducted a multicenter, single‐armed, phase II trial using two antiangiogenic cancer vaccines targeting VEGFR1 and VEGFR2 in addition to the KIF20A peptide. We attempted to evaluate the clinical benefit of the cancer vaccination in combination with gemcitabine. Chemotherapy naïve PC patients were enrolled to evaluate primarily the 1‐year survival rate, and secondarily overall survival (OS), progression free survival (PFS), response rate (RR), disease control rate (DCR) and the peptide‐specific immune responses. All enrolled patients received therapy without the HLA‐A information, and the HLA genotypes were used for classification of the patients. Between June 2012 and May 2013, a total of 68 patients were enrolled. No severe systemic adverse effects of Grade 3 or higher related to these three peptides were observed. The 1‐year survival rates between the HLA‐A*2402‐matched and ‐unmatched groups were not significantly different. In the HLA‐A*2402 matched group, patients showing peptide‐specific CTL induction for KIF20A or VEGFR1 showed a better prognosis compared to those without such induction (P = 0.023, P = 0.009, respectively). In the HLA‐A*2402‐matched group, the patients who showed a strong injection site reaction had a better survival rate (P = 0.017) compared to those with a weak or no injection site reaction. This phase II study demonstrated that this therapeutic peptide cocktail might be effective in patients who demonstrate peptide‐specific immune reactions although predictive biomarkers are needed for patient selection in its further clinical application. Peptide‐specific IFN‐γ response (induction of CTLs) for KIF20A, VEGFR1 and VEGFR2, and its correlation with prognosis in the HLA‐A*2402 matched group. The patients with a peptide‐specific IFN‐γ response for either KIF20A or VEGFR1 showed significantly better OS compared to those without an IFN‐γ response in the HLA‐A*2402 matched group (P = 0.023, P = 0.009, respectively).