Insulin, Glucagon and Somatostatin Content of the Non-Obese Diabetic (NOD) Mouse Pancreas and Plasma Virus Antibodies to Coxsackie B- and Reoviruses

• Published in: The Tohoku Journal of Experimental Medicine Vol. 146; no. 2; pp. 153 - 162
• Main Authors: TASAKA, YOSHIMASA, INOUE, YUKIKO, MARUMO, KOJI, MIZUNO, YOSHIATSU, HIRATA, YUKIMASA, AKAO, YORIYUKI
• Format: Journal Article
• Language: English
• Published: Japan Tohoku University Medical Press 1985
• Subjects: Animals; Antibodies, Viral - analysis; Blood Glucose - analysis; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - immunology; Diabetes Mellitus, Experimental - physiopathology; Enterovirus B, Human - immunology; Female; glucagon; Glucagon - analysis; Insulin - analysis; Male; Mice; Mice, Inbred Strains; NOD mouse; Pancreas - analysis; Pancreas - pathology; pancreas insulin; Pancreatic Hormones - analysis; plasma virus antibody; Reoviridae - immunology; somatostatin; Somatostatin - analysis
• ISSN: 0040-8727; 1349-3329
• DOI: 10.1620/tjem.146.153

TASAKA, Y., INOUE, Y., MARUMO, K., HIRATA, T. and AKAO, Y. Insulin, Glucagon and Somatostatin Content of the Non-Obese Diabetic (NOD) Mouse Pancreas and Plasma Virus Antibodies to Coxsackie B- and Reoviruses. Tohoku J. exp. Med., 1985, 146 (2), 153-162-The amounts of insulin, glucagon and somatostatin in the pancreas of NOD mice were determined and the results were compared with those of normal ICR-strain mice, and plasma antibodies to Coxsackie B-3 and reovirus types 1, 2 and 3 were measured. In the pancreas of NOD mice with fasting plasma glucose (FPG) less than 140mg/100ml, the insulin content of the male mice was similar to that of the normal controls, ranging to 3.55±0.31U/g wet weight of pancreas, but it was already significantly decreased to 0.85±0.52U/g in the female mice. In the NOD mice with FPG more than 201 mg/100ml, it was 0.002±0.001U/g. The glucagon content of the pancreas was 7.76±0.89μg/g in the normal controls and it was decreased slightly in the NOD mice, but the values among the NOD mice were not significantly different. Pancreas somatostatin showed a tendency to be higher in the NOD mice with FPG more than 201mg/100 ml. Histologically cell infiltration into the pancreatic islets was conspicious in the hyperglycemic NOD mice, but it was found even in the normoglycemic NOD. Plasma antibodies to Coxsackie B-3 Virus and reovirus types 1, 2 and 3 were not detected at any stage of FPG.