Two-phase appearance of oral epithelial dysplasia resulting from focal proliferation of parabasal cells and apoptosis of prickle cells
Background: One of the histologic characteristics of epithelial dysplasias of the oral mucosa is droplet‐shaped rete processes resulting from a solid proliferation of basaloid cells. These basaloid cells are suddenly changed into an overlay of parakeratotic cells. However, it is unknown how this ch...
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Published in | Journal of oral pathology & medicine Vol. 34; no. 3; pp. 140 - 149 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Munksgaard International Publishers
01.03.2005
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | Background: One of the histologic characteristics of epithelial dysplasias of the oral mucosa is droplet‐shaped rete processes resulting from a solid proliferation of basaloid cells. These basaloid cells are suddenly changed into an overlay of parakeratotic cells. However, it is unknown how this characteristic two‐phase appearance is generated.
Methods: Formalin‐fixed paraffin sections of the oral mucosal specimens with normal, hyperplastic, dysplastic epithelia and squamous cell carcinomas were examined for apoptosis by terminal deoxynucleotidyl transferase (TdT)‐mediated dUTP nick‐end labeling (TUNEL) method and for lymphoid cells by immunohistochemistry.
Results: Apoptotic cells were only located in the keratinized layer of normal/hyperplastic epithelia. However, in epithelial dysplasias, apoptotic cells were scattered in the middle or even in the lower parts of the epithelial layer with frequent vacuolation changes of epithelial cells. Within the epithelial layer of dysplasias, there were increased number of lymphocytes, which were immunopositive for CD45RO, CD8, and CD57‐ and CD68‐immunopositive (+), S‐100 protein‐positive and major histocompatibility complex (MHC) class II‐positive monocytic lineages. They increased in number with the severity of dysplastic degrees, and they were often located in the vicinity of apoptotic epithelial cells, but decreased in carcinomas in situ and invasive carcinomas, which contained fewer numbers of apoptotic figures.
Conclusion: The findings indicate that intraepithelial infiltrations of both cytotoxic T cells and natural killer cells are closely related to the apoptotic phenomena of prickle cells, which may result in the characteristic ‘two‐phase appearance’ of epithelial dysplasia. |
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Bibliography: | ArticleID:JOP283 ark:/67375/WNG-P4BFDQWK-2 istex:F1E66F8EDE568347A1D448D6B7EDE106607035A4 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0904-2512 1600-0714 |
DOI: | 10.1111/j.1600-0714.2004.00283.x |