Insulin-like growth factor binding protein-1 at mouse neuromuscular synapses

The insulin-like growth factor (IGF) signaling system includes the growth factors and their cell surface receptors, along with circulating IGF binding proteins (IGFBPs) that may alter and modulate the action of these neurotrophic hormones. These IGFBPs, along with IGFs and receptors, have been detec...

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Bibliographic Details
Published inSynapse (New York, N.Y.) Vol. 17; no. 4; p. 225
Main Authors Ma, J, Yang, S X, Ho, G J, Festoff, B W
Format Journal Article
LanguageEnglish
Published United States 01.08.1994
Subjects
Animals
Blotting, Western
Carrier Proteins - metabolism
Cattle
Electrophoresis, Polyacrylamide Gel
Female
Humans
Immunohistochemistry
Insulin-Like Growth Factor Binding Protein 1
Mice
Mice, Inbred BALB C
Neuromuscular Junction - metabolism
Receptors, Cholinergic - metabolism
Somatomedins - metabolism
Synapses - metabolism
Tissue Distribution
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Summary:The insulin-like growth factor (IGF) signaling system includes the growth factors and their cell surface receptors, along with circulating IGF binding proteins (IGFBPs) that may alter and modulate the action of these neurotrophic hormones. These IGFBPs, along with IGFs and receptors, have been detected in various tissues including the brain. In this study, using polyclonal antibody to human IGFBP-1 or bovine IGFBP-2, we found that mouse muscle extracts contain similar-sized proteins that cross-react with these antibodies on Western immunoblots. After establishing that these antibodies reacted with the homologous murine IGFBPs, we performed immunocytochemistry to demonstrate the localization of IGFBP-1 at the neuromuscular junction, a model nicotinic, cholinergic synapse, as well as within intramuscular nerves. IGFBP-2, a distinct macromolecule, is present on the surface of muscle fibers and is not present within synapses or nerves.
ISSN:0887-4476
1098-2396
DOI:10.1002/syn.890170403