Duodenal follicular lymphoma: Comprehensive gene expression analysis with insights into pathogenesis

• Published in: Cancer science Vol. 105; no. 5; pp. 608 - 615
• Main Authors: Takata, Katsuyoshi, Tanino, Motohiko, Ennishi, Daisuke, Tari, Akira, Sato, Yasuharu, Okada, Hiroyuki, Maeda, Yoshinobu, Goto, Naoe, Araki, Hiroshi, Harada, Mai, Ando, Midori, Iwamuro, Masaya, Tanimoto, Mitsune, Yamamoto, Kazuhide, Gascoyne, Randy D., Yoshino, Tadashi
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2014; BlackWell Publishing Ltd
• Subjects: Breast cancer; Cadherins - biosynthesis; Cadherins - genetics; CCL20; CCL20 protein; CCR6 protein; Cell Transformation, Neoplastic - genetics; Chemokine CCL20 - genetics; Chromosomes; Dendritic cells; Dendritic Cells - immunology; DNA microarrays; Down-Regulation; duodenal follicular lymphoma; Duodenal Neoplasms - genetics; Duodenal Neoplasms - metabolism; Duodenum; Duodenum - pathology; Gastrointestinal tract; Gene expression; gene expression profile; Gene Expression Profiling; Humans; Immunofluorescence; Immunoglobulins - genetics; Immunological memory; Lymphocytes B; Lymphoma; Lymphoma, B-Cell, Marginal Zone - genetics; Lymphoma, B-Cell, Marginal Zone - metabolism; Lymphoma, Follicular - genetics; Lymphoma, Follicular - metabolism; MAdCAM‐1; MALT lymphoma; Molecular Sequence Data; Mucoproteins - genetics; Mucosal-associated lymphoid tissue; Nervous system; Oligonucleotide Array Sequence Analysis; Original; Paraffin; Pathogenesis; Patients; Proteins; Protocadherin; Receptors, CCR6 - biosynthesis; Receptors, CCR6 - genetics; RNA polymerase; Small intestine; Software; Studies; Tumorigenesis; Up-Regulation; United States > US; Japan; MAdCAM-1; gene expression profile; MALT lymphoma; CCL20; duodenal follicular lymphoma
• ISSN: 1347-9032; 1349-7006
• DOI: 10.1111/cas.12392

Follicular lymphoma (FL) of the gastrointestinal tract, particularly duodenal follicular lymphoma (DFL), is a rare variant of FL with indolent clinical behavior, and this disease is included in the 2008 World Health Organization classification system. In contrast to nodal follicular lymphoma (NFL), DFL occurs most frequently in the second part of the duodenum, lacks follicular dendritic cell meshworks and has memory B‐cell characteristics. However, its molecular pathogenesis is still unclear. In the present study, we examined 10 DFL, 18 NFL and 10 gastric MALT lymphoma samples using gene expression analysis. Quantitative RT‐PCR experiments and immunohistochemical analysis for 72 formalin‐fixed, paraffin‐embedded tissues from an independent series, including 32 DFL, 19 gastric MALT lymphoma and 27 NFL samples, were performed for validation of microarray data. Gene expression profiles of the three lymphoma types were compared using 2918 differentially expressed genes (DEG) and results suggested that DFL shares characteristics of MALT lymphoma. Among these DEG, CCL20 and MAdCAM‐1 were upregulated in DFL and MALT but downregulated in NFL. In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL. Quantitative RT‐PCR and immunohistochemical studies demonstrated concordant results. Double immunofluorescence studies revealed that CCL20 and CCR6 were co‐expressed in both DFL and MALT. We hypothesize that increased expression of CCL20 and MAdCAM‐1 and co‐expression of CCL20 and CCR6 may play an important role in tumorigenesis. In the present study, we examined 10 DFL, 18 NFL, 10 gastric MALT lymphoma samples by gene expression analysis. Gene expression profiles of the 3 lymphoma types were compared using 2918 differentially expressed genes (DEGs) and results suggested that DFL shares characteristics of MALT lymphoma. Increased expression of CCL20 and MAdCAM‐1 and co‐expression of CCL20 and CCR6 may play an important role in tumorigenesis in DFL.