Molecular characterization of watermelon chlorotic stunt virus (WmCSV) from Palestine

The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.)...

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Published inViruses Vol. 6; no. 6; pp. 2444 - 2462
Main Authors Ali-Shtayeh, Mohammed S, Jamous, Rana M, Mallah, Omar B, Abu-Zeitoun, Salam Y
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 20.06.2014
MDPI
Subjects
Base Sequence
Begomovirus
Begomovirus - classification
Begomovirus - genetics
Biodiversity
Citrullus - virology
Citrullus lanatus
Cloning, Molecular
Cucumbers
Cucumis sativus
Disease
DNA, Viral
Gene Order
Genetic testing
Genetic Vectors
Genome, Viral
Genomes
Infections
Molecular Sequence Data
Palestine
Phenotype
Phylogeny
Plant Diseases - virology
Plant Leaves - virology
Polymerase chain reaction
RCA
Sequence Alignment
Sequence Analysis, DNA
SLCV
Viruses
watermelon
Watermelon chlorotic stunt virus
WmCSV
Lebanon
Israel
Iran
Oman
Sudan
Yemen
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Summary:The incidence of watermelon chlorotic stunt disease and molecular characterization of the Palestinian isolate of Watermelon chlorotic stunt virus (WmCSV-[PAL]) are described in this study. Symptomatic leaf samples obtained from watermelon Citrullus lanatus (Thunb.), and cucumber (Cucumis sativus L.) plants were tested for WmCSV-[PAL] infection by polymerase chain reaction (PCR) and Rolling Circle Amplification (RCA). Disease incidence ranged between 25%-98% in watermelon fields in the studied area, 77% of leaf samples collected from Jenin were found to be mixed infected with WmCSV-[PAL] and SLCV. The full-length DNA-A and DNA-B genomes of WmCSV-[PAL] were amplified and sequenced, and the sequences were deposited in the GenBank. Sequence analysis of virus genomes showed that DNA-A and DNA-B had 97.6%-99.42% and 93.16%-98.26% nucleotide identity with other virus isolates in the region, respectively. Sequence analysis also revealed that the Palestinian isolate of WmCSV shared the highest nucleotide identity with an isolate from Israel suggesting that the virus was introduced to Palestine from Israel.
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These authors contributed equally to this work.
ISSN:1999-4915
1999-4915
DOI:10.3390/v6062444