Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo
Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and early cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed . Eleven females were used as embryo dono...
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Published in | Frontiers in cell and developmental biology Vol. 10; p. 863700 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers media
21.03.2022
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and early cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed
. Eleven females were used as embryo donors and pools of embryos at the 4-6 cell, 8-12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with label free quantitative mass spectrometry. A total of 2,757 proteins were identified, of which 1,950 were quantitatively analyzed. Principal component analysis of data showed a clear separation of embryo pools according to their developmental stage. The hierarchical clustering of differentially abundant proteins evidenced a first cluster of 626 proteins that increased in abundance during development and a second cluster of 400 proteins that decreased in abundance during development, with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis, whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. The pairwise comparison between stages allowed us to identify specific protein interaction networks and metabolic pathways at the time of EGA, morula compaction and blastocyst formation. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed
. These data provide a number of protein candidates that will be useful for further mechanistic and functional studies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC8979002 Anette Kristine Krogenæs, Norwegian University of Life Sciences, Norway Sofia Diaz-Lundahl, Norwegian University of Life Sciences, Norway, in collaboration with reviewer AK This article was submitted to Molecular and Cellular Reproduction, a section of the journal Frontiers in Cell and Developmental Biology These authors have contributed equally to this work and share first authorship Il-Keun Kong, Gyeongsang National University, South Korea Edited by: João Ramalho-Santos,University of Coimbra,Portugal Reviewed by: Bernard Roelen, Utrecht University, Netherlands |
ISSN: | 2296-634X 2296-634X |
DOI: | 10.3389/fcell.2022.863700 |