Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

• Published in: Diagnostics (Basel) Vol. 12; no. 1; p. 208
• Main Author: Sanchini, Andrea
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.01.2022; MDPI
• Subjects: antibiotic resistance; Antibiotics; Antimicrobial agents; antimicrobial susceptibility testing; Automation; Bacteria; Drug resistance; Keywords; Medical Subject Headings-MeSH; molecular methods; MRSA; Nosocomial infections; Pathogens; Penicillin; Pneumonia; Point of care testing; rapid detection; Reproducibility; Review; Staphylococcus aureus; Staphylococcus infections; Vocabularies & taxonomies; molecular methods; antimicrobial susceptibility testing; phenotypic methods; antimicrobial stewardship; antibiotic resistance; resistance detection; MRSA; point of care; rapid detection; Staphylococcus aureus
• ISSN: 2075-4418; 2075-4418
• DOI: 10.3390/diagnostics12010208

Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of infections in humans, such as skin and soft tissue infections, pneumonia, food poisoning or sepsis. Historically, S. aureus was able to rapidly adapt to anti-staphylococcal antibiotics and become resistant to several classes of antibiotics. Today, methicillin-resistant S. aureus (MRSA) is a multidrug-resistant pathogen and is one of the most common bacteria responsible for hospital-acquired infections and outbreaks, in community settings as well. The rapid and accurate diagnosis of antimicrobial resistance in S. aureus is crucial to the early initiation of directed antibiotic therapy and to improve clinical outcomes for patients. In this narrative review, I provide an overview of recent phenotypic and molecular diagnostic methods for antimicrobial resistance detection in S. aureus, with a particular focus on MRSA detection. I consider methods for resistance detection in both clinical samples and isolated S. aureus cultures, along with a brief discussion of the advantages and the challenges of implementing such methods in routine diagnostics.