miR-30b-5p Downregulation as a Predictive Biomarker of Coronary In-Stent Restenosis

• Published in: Biomedicines Vol. 9; no. 4; p. 354
• Main Authors: Gutierrez-Carretero, Encarnación, Mayoral-González, Isabel, Jesús Morón, Francisco, Fernández-Quero, Mónica, Domínguez-Rodríguez, Alejandro, Ordóñez, Antonio, Smani, Tarik
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.03.2021; MDPI
• Subjects: Angina pectoris; Angiography; biomarker; Biomarkers; Cardiovascular disease; Cell differentiation; Cell migration; Cell proliferation; Cholesterol; Coronary vessels; Drug delivery; Endothelial cells; Gene expression; Heart failure; Implants; in-stent restenosis; Medical imaging; MicroRNAs; miRNA; miRNAs; Primary Coronary Intervention; Restenosis; Smooth muscle; Software; Stents; Thermal cycling; United States > US; Germany; Primary Coronary Intervention; miRNAs; biomarker; in-stent restenosis
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines9040354

In-stent restenosis (ISR) is one of the main limitations of percutaneous coronary intervention (PCI) therapy with drug-eluting stents (DES) implantation. The aim of this study was to determine if circulating microRNAs (miRNAs) have diagnostic capability for determining ISR in a cohort of matched patients. Blood samples were collected from 55 patients who underwent previously PCI and were readmitted for a new coronary angiography. Patients were divided into subgroups comprising patients who presented ISR or not (non-ISR). A microarray analysis determined that up to 49 miRNAs were differentially expressed between ISR and non-ISR patients. Of these, 10 miRNAs are related to vascular smooth muscle and endothelial cells proliferation, migration, and differentiation, well-known hallmarks of vascular remodeling. Additionally, we identified that the expression of miR-30b-5p is significantly lower in serum samples of ISR patients, as compared to non-ISR. A further analysis demonstrated that miR-30b-5p provides better values of the receiver operator characteristic curve than other miRNAs and biochemical parameters. Finally, the in-silico analysis suggests that miR-30b-5p is predicted to target 62 genes involved in different signaling pathways involved in vascular remodeling. In conclusion, we determined for the first time that circulating mi-R30b-5p can reliably prognose restenosis in patient with implanted DES, which could be potentially helpful in the establishment of an early diagnosis and therapy of ISR.