Bone Regeneration Effect of Hyperbaric Oxygen Therapy Duration on Calvarial Defects in Irradiated Rats

• Published in: BioMed research international Vol. 2019; no. 2019; pp. 1 - 9
• Main Authors: Chung, Moon-Kyu, Park, Young-Bum, Park, Wonse, Kim, Changdae, Park, Kyeong-Mee, Kim, Sungtae
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2019; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Anesthesia; Angiogenesis; Animals; Bone grafts; Bone growth; Bone histomorphometry; Bone Regeneration - radiation effects; Bone remodeling; Calcium phosphates; Collagen; Computed tomography; Defects; Grafting; Grafts; Histology; Hyperbaric oxygen therapy; Hyperbaric Oxygenation; Hypoxia; Ionizing radiation; Laboratory animals; Male; Oxygen; Oxygen therapy; Radiation; Radiation Injuries, Experimental - diagnostic imaging; Radiation Injuries, Experimental - metabolism; Radiation Injuries, Experimental - pathology; Radiation Injuries, Experimental - therapy; Radiography; Rats; Rats, Sprague-Dawley; Regeneration; Regeneration (physiology); Skin & tissue grafts; Skull; Skull - diagnostic imaging; Skull - injuries; Skull - metabolism; Skull - pathology; Substitute bone; Surgery; Therapy; Transplants & implants; X-Ray Microtomography; X-Rays - adverse effects; South Korea; Germany
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2019/9051713

Objective. In this study, we evaluated changes in bone remodeling in an irradiated rat calvarial defect model according to duration of hyperbaric oxygen therapy. Materials and Methods. The 28 rats were divided into four groups. Radiation of 12 Gy was applied to the skull, and 5-mm critical size defects were formed on both sides of the skull. Bone grafts were applied to one side of formed defects. From the day after surgery, HBO was applied for 0, 1, and 3 weeks. At 6 weeks after bone graft, experimental sites were removed and analyzed for radiography, histology, and histomorphometry. Results. Micro-CT analysis showed a significant increase in new bone volume in the HBO-3 group, with or without bone graft. When bone grafting was performed, BV, BS, and BS/TV all significantly increased. Histomorphometric analysis showed significant increases in %NBA and %BVN in the HBO-1 and HBO-3 groups, regardless of bone graft. Conclusion. Hyperbaric oxygen therapy was effective for bone regeneration with only 1 week of treatment.