In vivo evoked specific cell mediated cytotoxicity in carp ( Cyprinus carpio L.) uses mainly a perforin/granzyme-like pathway

• Published in: Fish & shellfish immunology Vol. 20; no. 1; pp. 113 - 117
• Main Authors: Companjen, Arjen, Heinhuis, Bas, Aspers, Kelly, Rombout, Jan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 2006
• Subjects: allogeneic targets; Animals; Calcium - immunology; Carp; Carps - immunology; Cell Line, Tumor; Cell mediated cytotoxicity; Cytotoxicity Tests, Immunologic - methods; Cytotoxicity Tests, Immunologic - veterinary; Cytotoxicity, Immunologic - immunology; Egtazic Acid; fas ligand; fish; ginbuna crucian carp; Granzyme; ictalurus-punctatus; identification; induction; L-Lactate Dehydrogenase; leukocytes; mechanisms; Membrane Glycoproteins - immunology; Perforin; Pore Forming Cytotoxic Proteins; sparus-aurata l; Granzyme; Cell mediated cytotoxicity; Carp; Perforin
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2005.03.009

A specific cytotoxicity assay has been developed for carp using the Epithelioma Papulosum Cyprini (EPC) cell line as target cells and the release of lactate dehydrogenase (LDH) as read out system. Non-specific cytotoxicity against EPC was not observed, but animals immunized with EPC clearly showed specific killing by effector cells present in kidney, blood and spleen. This killing was strongly calcium dependent, suggesting the utilization of a perforin/granzyme-like pathway. However, blocking with EGTA was not complete indicating the existence of other additional killing routes.