Association of transforming growth factor-β1 gene polymorphisms with genetic susceptibility to acute myocardial infarction

• Published in: The American journal of the medical sciences Vol. 342; no. 5; p. 365
• Main Authors: Najar, Reza Akbarzadeh, Ghaderian, Sayyed Mohammad Hossein, Panah, Akram Sadat Tabatabaei
• Format: Journal Article
• Language: English
• Published: United States 01.11.2011
• Subjects: Acute Disease; Alleles; Female; Genetic Predisposition to Disease; Genotype; Humans; Iran; Leukocytes, Mononuclear - physiology; Male; Middle Aged; Myocardial Infarction - genetics; Polymorphism, Genetic; Risk Factors; Transforming Growth Factor beta1 - blood; Transforming Growth Factor beta1 - genetics; Iran
• ISSN: 1538-2990
• DOI: 10.1097/MAJ.0b013e318215908a

Transforming growth factor beta 1 (TGF-β1) gene plays an important role in acute myocardial infarction (AMI); however, little is known about the relation of variations within the gene and risk of cardiovascular diseases. In this study, the authors evaluated the influence of TGF-β1 polymorphisms on the onset and progression of AMI in Iranian patients comparing with healthy individuals. Genomic DNA and peripheral blood mononuclear cells of 900 enrolled patients with AMI and 900 control subjects were extracted. The -509 C/T, 868T/C, 913G/C and 11929C/T TGF-β1 polymorphisms were detected. The messenger RNA (mRNA) expression and serum levels of TGF-β1 were analyzed by real-time reverse-transcriptase polymerase chain reaction and ELISA, respectively. The frequency of "T" allele in -509 C/T, "C" allele in 868T/C, "C" allele in 913G/C and "T" allele in 11929C/T polymorphisms were significantly higher in the patients than control subjects (P < 0.001). There were significant differences in circulating levels of TGF-β1 in the patients than in control subjects (P < 0.001). These concentrations are associated with its gene polymorphism. The mRNA expression levels of TGF-β1 were significantly higher in the patient serums compared with controls (P < 0.001). Our results confirmed the association between the TGF-β1 polymorphisms and risk of AMI, which suggest that genetic polymorphisms in TGF-β1 might be helpful for determining susceptibility to AMI in Iranian patients. There are also significant relationship between serum TGF-β1 and occurrence of AMI. In addition, susceptibility to AMI might be related to TGF-β1 gene expression, which affects its serum levels.