Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae
Infections associated with antimicrobial-resistant bacteria now represent a significant threat to human health using conventional therapy, necessitating the development of alternate and more effective antibacterial compounds. Silver nanoparticles (Ag NPs) have been proposed as potential antimicrobia...
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Published in | Frontiers in microbiology Vol. 12; p. 638640 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media
15.02.2021
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Infections associated with antimicrobial-resistant bacteria now represent a significant threat to human health using conventional therapy, necessitating the development of alternate and more effective antibacterial compounds. Silver nanoparticles (Ag NPs) have been proposed as potential antimicrobial agents to combat infections. A complete understanding of their antimicrobial activity is required before these molecules can be used in therapy. Lysozyme coated Ag NPs were synthesized and characterized by TEM-EDS, XRD, UV-vis, FTIR spectroscopy, zeta potential, and oxidative potential assay. Biochemical assays and deep level transcriptional analysis using RNA sequencing were used to decipher how Ag NPs exert their antibacterial action against multi-drug resistant
MGH78578. RNAseq data revealed that Ag NPs induced a
bactericidal mechanism responsible for the inhibition of the type II fatty acid biosynthesis. Additionally, released Ag
generated oxidative stress both extra- and intracellularly in
. The data showed that
activity and oxidative stress cumulatively underpinned the antibacterial activity of Ag NPs. This result was confirmed by the analysis of the bactericidal effect of Ag NPs against the isogenic
MGH78578 Δ
mutant, which exhibits a compromised oxidative stress response compared to the wild type. Silver nanoparticles induce a
antibacterial action mechanism in multi-drug resistant
. This study extends our understanding of anti-
mechanisms associated with exposure to Ag NPs. This allowed us to model how bacteria might develop resistance against silver nanoparticles, should the latter be used in therapy. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Jong H. Kim, Agricultural Research Service, United States Reviewed by: Anima Nanda, Sathyabama Institute of Science and Technology, India; Sanjay Kumar Singh Patel, Konkuk University, South Korea ORCID: Vikram Pareek, orcid.org/0000-0003-3980-1238; Stéphanie Devineau, orcid.org/0000-0002-1133-5223; Sathesh K. Sivasankaran, orcid.org/0000-0003-3037-6001; Arpit Bhargava, orcid.org/0000-0001-6450-6551; Jitendra Panwar, orcid.org/0000-0002-0750-9745; Shabarinath Srikumar, orcid.org/0000-0003-3775-2831; Séamus Fanning, orcid.org/0000-0002-1922-8836 This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.638640 |