Characterization of Fusarium oxysporum isolates obtained from cucumber in China by pathogenicity, VCG, and RAPD

• Published in: Plant disease Vol. 88; no. 6; pp. 645 - 649
• Main Authors: VAKALOUNAKIS, D. J, WANG, Z, FRAGKIADAKIS, G. A, SKARACIS, G. N, LI, D.-B
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.06.2004
• Subjects: Biological and medical sciences; Cucumis sativus; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fusarium oxysporum; Pathogens; Phytopathology. Animal pests. Plant and forest protection; Asia; China; China, People's Rep; Plant pathology; Fusarium oxysporum; Microbiology; Plant pathogen; Cucurbitaceae; Cucumis sativus; Random amplified polymorphic DNA marker; Fungi; Pathogenicity; Dicotyledones; Angiospermae; Isolate; Spermatophyta; Fungi Imperfecti; Thallophyta
• ISSN: 0191-2917; 1943-7692
• DOI: 10.1094/pdis.2004.88.6.645

Thirty-four isolates of Fusarium oxysporum, obtained in China from cucumber plants showing either Fusarium wilt (F. oxysporum f. sp. cucumerinum) or root and stem rot (F. oxysporum f. sp. radicis-cucumerinum) symptoms, were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic DNA (RAPD). Of these, 23 isolates were identified by pathogenicity as F. oxysporum f. sp. cucumerinum, and one as F. oxysporum f. sp. radicis-cucumerinum, while 10 isolates were avirulent on cucumber, melon, sponge gourd, and pumpkin. The Chinese isolates of F. oxysporum f. sp. cucumerinum were assigned to RAPD groups III and XXI and to vegetative compatibility group (VCG) 0183, four new VCGs, 0184 to 0187, and a single-member VCG included in the artificial VCG 018-. The Chinese isolate of F. oxysporum f. sp. radicis-cucumerinum was assigned to RAPD group I and bridging VCG 0260/0261. The occurrence of F. oxysporum f. sp. radicis-cucumerinum on cucumber is reported for the first time in China.