Concentration of progesterone during the development of the ovulatory follicle: I. Ovarian and embryonic responses

• Published in: Journal of dairy science Vol. 94; no. 7; pp. 3342 - 3351
• Main Authors: Cerri, R.L.A., Chebel, R.C., Rivera, F., Narciso, C.D., Oliveira, R.A., Thatcher, W.W., Santos, J.E.P.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.07.2011; Elsevier
• Subjects: Animal productions; Animals; artificial insemination; Biological and medical sciences; blastomeres; blood; Cattle - blood; Cattle - growth & development; Cattle - physiology; corpus luteum; cows; dairy cow; Dinoprost - pharmacology; embryo quality; estradiol; Estradiol - blood; estrus; Estrus Synchronization - methods; Female; Fertility - drug effects; Fertility - physiology; Fertility Agents, Female - blood; Fertility Agents, Female - pharmacology; follicular development; Food industries; Fundamental and applied biological sciences. Psychology; gonadotropin-releasing hormone; Holstein; Insemination, Artificial - veterinary; lactation; Milk and cheese industries. Ice creams; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; ovulation; Parity; Pregnancy; progesterone; Progesterone - blood; Progesterone - pharmacology; reproduction; Terrestrial animal productions; ultrasonography; Vertebrates; embryo quality; dairy cow; progesterone; reproduction; Embryo; Farming animal; Dairy cattle; Concentration; Vertebrata; Reproduction; Mammalia; Quality; Dairy industry; Development; Artiodactyla; Progesterone; Ungulata
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2010-3734

Objectives were to evaluate the effects of differing progesterone concentrations during follicle development on follicular dynamics, fertilization, and embryo quality. Lactating Holstein cows (n=154) were assigned randomly to 1 of 2 treatments. Cows underwent a presynchronization of the estrous cycle composed of an injection of GnRH concurrently with the placement of a progesterone insert, an injection of PGF2α and insert removal 7 d later, and a second injection of GnRH 48h later (study d −16). All cows were then submitted to a hormonal protocol identical to the presynchronization program starting on d 7 of the estrous cycle (study d −9). Cows enrolled in the high progesterone (HP) treatment received no further treatment. Cows in the low progesterone (LP) treatment received additional PGF2α injections on study d −14, −13.5, and −13 and again on study d −9, −7, −6.5, and −6. Ovaries were evaluated by ultrasonography, and blood was sampled for concentrations of progesterone and estradiol throughout the study. Uteri were flushed 6 d after artificial insemination (AI) and recovered oocytes-embryos were evaluated. Concentrations of progesterone were less for LP cows from study d −7 to −2; concentrations of estradiol at PGF2α and at the last GnRH of synchronization were greater for LP than HP. The proportion of cows in estrus at AI was greater for LP than for HP (38.0 vs. 5.3%). Ovulatory follicles of LP cows had larger diameters at the injections of PGF2α (17.2 vs. 14.6mm) and final GnRH (19.4 vs. 16.9%) of the synchronization, which resulted in a larger diameter of the corpus luteum 6 d after AI (24.3 vs. 22.6mm). Double ovulation after the last GnRH of the synchronization was increased in LP (18.6%) compared with HP (4.5%). Fertilization rate was similar and averaged 82.7%. The proportion of embryos and oocytes-embryos classified as grades 1 and 2, proportion of degenerated embryos, and unfertilized-degenerated oocytes-embryos were not different between LP and HP. Number of blastomeres did not differ between LP and HP, but the proportion of live blastomeres tended to be less for LP than HP (94.2 vs. 98.7%). Reducing progesterone concentrations during the synchronization program altered concentrations of estradiol and follicular dynamics, but resulted in similar fertilization and only minor changes in embryo quality.