Uptake and accumulation of B-group vitamers in Saccharomyces cerevisiae in ethanol-stat fed-batch culture

• Published in: World journal of microbiology & biotechnology Vol. 30; no. 9; pp. 2351 - 2359
• Main Authors: Paalme, T, Kevvai, K, Vilbaste, A, Hälvin, K, Nisamedtinov, I
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.09.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Accumulation; Acids; Agricultural biotechnology; Applied Microbiology; Assimilation; Batch Cell Culture Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Culture; culture media; Culture Media - chemistry; Dosage; Environmental Engineering/Biotechnology; Enzymes; Ethanol; Ethanol - metabolism; Grain; Kinases; Life Sciences; Metabolism; Microbiology; Nicotinamide; Original Paper; pantothenic acid; Pyridoxine; Riboflavin; Saccharomyces cerevisiae; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Studies; thiamin; Thiamine; Uptakes; Vitamin B; Vitamin B Complex - metabolism; Vitamins; Yeast; Yeasts; Estonia; Fed-batch; Stable isotope dilution assay; LC–MS; B-group vitamins
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-014-1660-x

The uptake and accumulation of the B-group vitamins thiamine, riboflavin, nicotinamide, pantothenic acid and pyridoxine in Saccharomyces cerevisiae was studied by gradually increasing the specific dosage of vitamins in an ethanol-stat fed-batch culture. Thiamine, nicotinamide, pantothenic acid, and pyridoxine were almost completely taken up at low vitamin dosages. Thiamine was determined to be the major accumulating form of vitamin B₁ while most of the assimilated nicotinamide and pantothenic acid accumulated in cofactor forms. Despite the obvious uptake of pyridoxine, accumulation of B₆ vitamers was not observed. In contrast with the other vitamins studied, riboflavin began accumulating in the culture medium immediately after vitamin addition was initiated. By the end of the experiment, the apparent uptake of all vitamins exceeded their accumulation in the cells. Variations in the growth rate of yeast at different vitamin dosages demonstrate the importance of balancing the vitamins in the media during cultivation.