The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine

• Published in: Journal of Bacteriology Vol. 180; no. 9; pp. 2556 - 2559
• Main Authors: Schreve, J.L. (Hamilton College, Clinton, NY.), Sin, J.K, Garrett, J.M
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.05.1998
• Series: Note
• Subjects: ACIDE GLUTAMIQUE; ACIDO GLUTAMICO; ACTIVE TRANSPORT; ADN RECOMBINADO; ADN RECOMBINE; AGP1 GENE; AMINO ACID DERIVATIVES; Amino Acid Transport Systems; Amino Acid Transport Systems, Basic; Amino Acid Transport Systems, Neutral; Amino acids; Amino Acids, Dicarboxylic; Amino Acids, Dicarboxylic - pharmacology; AMINO COMPOUNDS; AMMONIA; Ammonia - pharmacology; AMMONIAC; AMONIACO; ASPARAGINA; ASPARAGINE; Asparagine - metabolism; Bacteria; Bacteriology; BETA GALACTOSIDASA; BETA GALACTOSIDASE; Biological Transport; CELL MEMBRANES; COMPOSE AMINE; COMPUESTOS DE AMINA; DISPONIBILIDAD DE NUTRIENTES; DISPONIBILITE D'ELEMENT NUTRITIF; drug effects; Enzymes; Eukaryotic Cells; EXPRESION GENICA; EXPRESSION DES GENES; GENE; GENE EXPRESSION; Gene Expression Regulation, Fungal; Gene Expression Regulation, Fungal - drug effects; GENES; Genes, Fungal; GENETIC REGULATION; GENETICA; GENETICS; GENETIQUE; GLUTAMIC ACID; GLUTAMINA; GLUTAMINE; Glutamine - metabolism; Kinetics; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Membrane Transport Proteins; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; metabolism; MUTANT; MUTANTES; MUTANTS; NUTRIENT AVAILABILITY; pharmacology; plasma membrane; PLASMA MEMBRANES; PROLINA; PROLINE; promoter regions; PROMOTERS; PROTEINAS; PROTEINE; PROTEINS; RECOMBINANT DNA; REPORTER GENES; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; STRUCTURAL GENES; TRANSCRIPCION; TRANSCRIPTION; transcription (genetics); UREA; UREE; YCC5 GENE; Yeasts
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.180.9.2556-2559.1998

The yeast YCC5 gene encodes a putative amino acid permease and is homologous to GNP1 (encoding a high-affinity glutamine permease). Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression