Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A

• Published in: Vaccine Vol. 24; no. 12; pp. 2110 - 2119
• Main Authors: Nakano, Hideki, Nagata, Toshi, Suda, Takafumi, Tanaka, Takao, Aoshi, Taiki, Uchijima, Masato, Kuwayama, Sachiko, Kanamaru, Noriko, Chida, Kingo, Nakamura, Hirotoshi, Okada, Masaji, Koide, Yukio
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 15.03.2006; Elsevier; Elsevier Limited
• Subjects: Animals; Antigen 85A; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Antigens, Bacterial - metabolism; Applied microbiology; Bacterial Vaccines - genetics; Bacterial Vaccines - immunology; Biological and medical sciences; Bone marrow; Colleges & universities; Cytotoxicity; Dendritic cells; Dendritic Cells - immunology; Deoxyribonucleic acid; DNA; Epitopes, T-Lymphocyte - immunology; Fundamental and applied biological sciences. Psychology; Gene expression; Immunization; Infections; Listeria; Lymphocytes; Mice; Mice, Inbred BALB C; Microbiology; Mycobacterium tuberculosis; Mycobacterium tuberculosis - immunology; Peptides; Proteins; Retroviridae - genetics; T-Lymphocytes, Cytotoxic - immunology; Transduction, Genetic; Tuberculosis; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Antigen 85A; Dendritic cells; Mycobacterium tuberculosis; Dendritic cell; Immunization; Antigenic determinant; Cytotoxicity; Cellular immunity; Vaccine; Antigen; Gene; Mycobacteriales; T-Lymphocyte; Mycobacteriaceae; Bacteria; Actinomycetes; Cytotoxic T lymphocyte
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2005.11.026

In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-γ production from both CD4 + and CD8 + T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-γ-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance.