Low-level laser irradiation facilitates fibronectin and collagen type I turnover during tooth movement in rats

• Published in: Lasers in medical science Vol. 25; no. 1; pp. 25 - 31
• Main Authors: Kim, Yong-Deok, Kim, Seong-Sik, Kim, Seok-Jun, Kwon, Dae-Woo, Jeon, Eun-Suk, Son, Woo-Sung
• Format: Journal Article
• Language: English
• Published: London Springer-Verlag 01.01.2010; Springer Nature B.V
• Subjects: Animals; Cellular biology; Clinical outcomes; Collagen Type I - metabolism; Collagen Type I - radiation effects; Dental care; Dentistry; Fibronectins - metabolism; Fibronectins - radiation effects; Immunohistochemistry; Lasers; Lasers, Semiconductor - therapeutic use; Low-Level Light Therapy - methods; Male; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Periodontium - anatomy & histology; Periodontium - metabolism; Periodontium - radiation effects; Photonics; Proteins; Quantum Optics; Rats; Rats, Sprague-Dawley; Rodents; Teeth; Time Factors; Tissues; Tooth Movement Techniques - methods; LLLT; Collagen type I; Tooth movement; Diode laser; Fibronectin; Wavelength
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-008-0585-8

The aim of this study was to investigate the effects of low-level laser (LLL) irradiation on the turnover of fibronectin and collagen type I in periodontal tissue during tooth movement in rats by immunohistochemistry. Thirty male Sprague–Dawley rats aged 15 weeks were assigned to either an experimental group ( n  = 15) that underwent LLL irradiation during tooth movement, or a control group ( n  = 15). In the experimental group, the gallium–aluminum–arsenide (Ga-Al-As) diode LLL (wavelength 808 nm; output 96 mW) was used to irradiate three areas on both the palatal side and the labial side of the maxillary incisor. The radiation was administered by the contact method for 10 s at 0.83 J/cm 2 energy dose, once a day for 7 days. Total energy dose over the complete schedule was 34.86 J/cm 2 . The animals were killed on days 1, 3, 7, 14 and 21. There was no difference between the two groups in the amount of tooth movement. The immunohistochemistry results showed that the expression of fibronectin and collagen type I in the experimental group had significantly increased from day 1, with a more even distribution than in the control group, and that this difference was maintained until the end of the experiment. These results suggest that LLL irradiation facilitates the reorganization of the connective tissues during tooth movement in rats.