Discovery of MK8383s with Antifungal Activity from Mangrove Endophytic Fungi Medicopsis sp. SCSIO 40440 Against Fusarium Wilt of Banana

• Published in: Marine drugs Vol. 23; no. 2; p. 88
• Main Authors: Zhou, Tianyu, Qiao, Yulei, Wang, Lu, Li, Zifeng, Zhang, Haibo, Zhang, Liping, Liao, Shengrong, Li, Minhui, Zhang, Changsheng, Zhang, Wenjun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2025; MDPI
• Subjects: antifungal activities against Foc TR4; Antifungal activity; Antifungal agents; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Bananas; Bioactive compounds; Chromatography; decalin carboxylic acids; Diffraction; Drug Discovery; Endophytes; Endophytes - chemistry; Enzymes; Fermentation; Fruit industry; Fruits; Fungi; Fungicides; Fusarium; Fusarium - drug effects; Fusarium - pathogenicity; Fusarium oxysporum; Hygromycin; Hygromycin B; mangrove endophytic fungi; Mangroves; Metabolites; Microbial Sensitivity Tests; Musa - microbiology; Natural products; Nystatin; Plant Diseases - microbiology; Plant Diseases - prevention & control; Single crystals; Spores; Virulence; Wilt; X-ray diffraction; X-ray diffraction analysis; X-rays; China; antifungal activities against Foc TR4; decalin carboxylic acids; mangrove endophytic fungi
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md23020088

Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (TR4), poses a severe threat to the global banana industry. The screening of endophytic fungi from the mangrove plant led to the identification of Medicopsis sp. SCSIO 40440, which exhibited potent antifungal activity against Fusarium. The further fraction of the extract yielded ten compounds, including MK8383 (1) and nine new analogues, MK8383s B-J (2–10). The structures of 1–10 were elucidated using extensive spectroscopic data and single-crystal X-ray diffraction analysis. In vitro antifungal assays revealed that 1 showed strongly antifungal activities against Foc TR4, with an EC50 of 0.28 μg/mL, surpassing nystatin and hygromycin B (32 and 16 μg/mL, respectively). Pot experiments showed that 1 or spores of SCSIO 40440 could significantly reduce the virulence of Foc TR4 on Cavendish banana.