Altered cervicovaginal microbiota in premenopausal ovarian cancer patients

• Published in: Gene Vol. 811; p. 146083
• Main Authors: Morikawa, Asuka, Kawabata, Ayako, Shirahige, Katsuhiko, Akiyama, Tetsu, Okamoto, Aikou, Sutani, Takashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2022
• Subjects: 16S rRNA gene sequencing; Adult; Aged; Aged, 80 and over; Bacterial Typing Techniques - methods; Biomarker; Biomarkers; Carcinoma, Ovarian Epithelial - microbiology; Case-Control Studies; Cervix Uteri - microbiology; DNA, Bacterial; early diagnosis; epithelium; Female; females; genes; Human microbiome; Humans; Japan; Lactobacillus - classification; Lactobacillus - genetics; Metagenome; microbiome; Microbiota; microorganisms; Middle Aged; ovarian neoplasms; Ovarian Neoplasms - microbiology; Postmenopause; Premenopause; RNA, Ribosomal, 16S; Vagina - microbiology; Vaginal microbiome; Young Adult; Japan; MOC; PCoA; Biomarker; CST; rRNA; SOC; ROC; Vaginal microbiome; 16S rRNA gene sequencing; AUC; Human microbiome; OCCC; OTU; OC
• ISSN: 0378-1119; 1879-0038; 1879-0038
• DOI: 10.1016/j.gene.2021.146083

Nearly three hundred thousand female patients are diagnosed with ovarian cancer in the world annually, and this number shows an increasing trend. However, characteristic symptoms caused by ovarian cancer are so few that early diagnosis remains challenging, and an effective screening method has not yet been established. Here, we conducted a case-control study in Japan to analyze the association between cervicovaginal microbiome and ovarian cancer, using 16S rRNA amplicon sequencing. Analysis of DNA extracted from cervical smear samples revealed Lactobacillus-dominant and Lactobacillus-deficient, highly-diversified bacterial communities in premenopausal and postmenopausal healthy controls, respectively, as reported for vaginal microbiota previously. We found that cervicovaginal microbiota in ovarian cancer patients, regardless of their menopausal status, were frequently a diversified community and similar to those in healthy subjects at postmenopausal ages. The diverse microbiota was associated with the major histotypes of epithelial ovarian cancer, including serous ovarian cancer and ovarian clear cell cancer. The present study implies the potential of a cervicovaginal microbiome biomarker in screening ovarian cancer in premenopausal women.